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| Product Name | Ultra-Pure DMSO for Cell Biology (≥99.9%, Hybri-Max Equivalent) |
| Catalog No. | ATR-SDS-0014 |
| Description | A dimethyl sulfoxide preparation of ≥99.9% purity specifically tested for suitability in cell biology applications including cryopreservation, drug solubilization, and cell-based assay compound delivery. The product undergoes additional purification and quality testing beyond standard reagent-grade DMSO, including cytotoxicity screening and HPLC purity verification. Low water content and absence of peroxides ensure reproducible solubilization of hydrophobic compounds without oxidative degradation. |
| Intended Use | Cryopreservation medium preparation and solubilization of hydrophobic compounds for cell-based assays. |
| Principle / Technology | Polar aprotic solvent enhancing cell membrane permeability for cryoprotectant and compound delivery |
| Detection Method | Cell viability assessment post-thaw; compound solubility verification by spectrophotometry |
| Sample Type | Mammalian cell lines for cryopreservation; hydrophobic small molecule compounds for cell treatment |
| Performance Range / Specifications | Purity: ≥99.9% by GC; water content ≤0.1%; freezing point: 18.5 °C |
| Sensitivity / LOD | Cell viability post-cryopreservation: ≥90% recovery of viable cells when used in standard freezing medium |
| Specificity | Meets cell culture specifications; peroxides below detection limit |
| Reaction Conditions / Protocol | Use at 5–10% v/v in cell freezing medium; typical compound stock concentration 10–100 mM; final culture DMSO concentration ≤0.5% v/v |
| Components / Formulation | Dimethyl sulfoxide, ≥99.9%, Hybri-Max tested, in amber glass bottle under nitrogen |
| Storage Conditions | Room temperature, protected from moisture; store under dry inert gas after opening |
| Shelf Life | 36 months in sealed container |
| Package Specifications | 50 mL, 100 mL, 500 mL |
| Product Form | Liquid, colorless, hygroscopic |
| Quality Control | Each lot tested for cytotoxicity on HeLa and HEK293 cells (IC50 ≥5% v/v, 24 hr exposure); HPLC purity ≥99.9% |
| Key Features | Extra purification beyond reagent grade ensures no batch-to-batch variability in cell-based assay performance |
| Purity | ≥99% by GC or HPLC; suitable for molecular biology applications |
| Concentration | As specified; typically neat or concentrated solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | Not applicable — organic solvent |
| Shipping Conditions | Ambient temperature; flammable liquids shipped per dangerous goods regulations |
| Expiration Date / Stability | 24–36 months in sealed container under inert atmosphere |
| Regulatory / Compliance | Research use; hazardous material classification per GHS; SDS provided |
| Compatibility | Compatible with standard laboratory glassware and polypropylene vessels |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | Handle in fume hood; flammable; avoid contact with oxidizers; store away from ignition sources |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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