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| Product Name | Dimethylformamide, Anhydrous, ≥99.8%, ACS Grade |
| Catalog No. | ATR-SDS-0024 |
| Description | Anhydrous N,N-dimethylformamide (DMF) purified to ACS specifications for use as a polar aprotic solvent in peptide synthesis, organic reactions, and as a solvent for water-insoluble compounds in biochemical assays. Supplied over molecular sieves to maintain anhydrous condition, with water content guaranteed below 0.01%. This solvent is essential for dissolving hydrophobic peptides, fluorescent dyes, and small molecule inhibitors for cell-based assays. |
| Intended Use | Solvent for peptide coupling reactions; dissolution of hydrophobic fluorescent probes and dyes for cell labeling; small molecule inhibitor stock solution preparation; organic synthesis of bioconjugates; HPLC mobile phase modifier. |
| Principle / Technology | Polar aprotic organic solvent with high dielectric constant that dissolves a wide range of polar and non-polar compounds while being miscible with water and most organic solvents |
| Detection Method | Use as received under anhydrous conditions; handle under nitrogen or argon atmosphere for moisture-sensitive applications |
| Components / Formulation | N,N-Dimethylformamide (CAS 68-12-2), ≥99.8% purity; molecular sieves 4A added as drying agent |
| Storage Conditions | Store at 15–25 °C in original container with septum seal; keep container tightly closed after use; flammable liquid — keep away from ignition sources |
| Shelf Life | 24 months from manufacture date in sealed container; retest after 6 months of opening for water content |
| Package Specifications | 100 mL, 500 mL, 1 L amber glass bottles with PTFE-lined septum caps; Sure/Seal packaging available |
| Product Form | Clear colorless liquid with characteristic amine odor; hygroscopic |
| Key Features | ACS grade purity ≥99.8%; water content <0.01%; evaporation residue <0.0005%; free amine (as dimethylamine) <0.001%; meets ACS, Reagent Ph.Eur., and USP specifications for chemical purity; packaged under dry nitrogen |
| Purity | ≥99.8% by GC; water <0.01% by Karl Fischer; acidity <0.0005 meq/g; free amine <0.001% |
| Concentration | As specified; typically neat or concentrated solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | 73.09 g/mol |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | Not applicable — organic solvent |
| Shipping Conditions | Ambient temperature; flammable liquid Class 3, UN 2265; ship per IATA/ADR regulations |
| Expiration Date / Stability | 24 months in sealed original container; after opening, retest water content every 6 months; discard if water exceeds 0.05% for anhydrous applications |
| Regulatory / Compliance | For laboratory and research use only; CAS 68-12-2; GHS02/GHS07/GHS08 classified; signal word: Danger; H226/H312/H319/H332/H360D; UN 2265, Class 3 |
| Compatibility | Compatible with glass, PTFE, and stainless steel; attacks many plastics including PVC, polycarbonate, and some grades of polyethylene and polypropylene at prolonged contact; use glass or PTFE for storage |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For dissolving cell-permeable fluorescent dyes (e.g., Fluo-4 AM, Fura-2 AM), prepare 1–5 mM stock in anhydrous DMF. Aliquot and store at -20 °C with desiccant. DMF is a reproductive toxin — always handle in a chemical fume hood with appropriate PPE including nitrile gloves (note: DMF penetrates latex). For peptide synthesis, use fresh DMF with low amine content. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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