Guanidinium Isothiocyanate Lysis Solution, 4 M

Name: Guanidinium Isothiocyanate Lysis Solution, 4 M
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Cat.No: ATR-SDS-0013 Datasheet

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Product Name	Guanidinium Isothiocyanate Lysis Solution, 4 M
Catalog No.	ATR-SDS-0013
Description	A concentrated chaotropic lysis reagent containing 4 M guanidinium isothiocyanate for the rapid denaturation of proteins and inactivation of RNases in RNA extraction from cells and tissues.
Intended Use	Primary lysis and homogenization reagent in single-step RNA isolation methods, immediately denaturing cellular proteins and inactivating endogenous and environmental RNases upon contact.
Principle / Technology	Guanidinium isothiocyanate is a powerful chaotropic agent that disrupts hydrogen bonding in proteins, denaturing all cellular proteins including RNases within seconds of contact; beta-mercaptoethanol reduces disulfide bonds to ensure complete denaturation.
Detection Method	RNA integrity by Bioanalyzer RIN score, A260/A280 ratio, RT-PCR functional validation
Sample Type	Cultured cells, fresh and frozen tissues, bacterial pellets, yeast
Performance Range / Specifications	RNA yield 5–50 µg per 10^6 cells; RIN score >8.0 for properly handled fresh samples
Sensitivity / LOD	N/A
Specificity	N/A
Reaction Conditions / Protocol	Add 1 mL per 10^7 cells or 100 mg tissue; homogenize immediately; proceed to organic extraction or silica column purification.
Components / Formulation	Guanidinium isothiocyanate 4 M, sodium citrate pH 7.0 25 mM, sodium N-lauroylsarcosine 0.5%, beta-mercaptoethanol 100 mM (or DTT 10 mM)
Storage Conditions	15–30°C, protect from light; add reducing agent fresh before use
Shelf Life	12 months (without reducing agent)
Package Specifications	100 mL, 500 mL
Product Form	Liquid
Quality Control	RNase inactivation validation, RNA integrity testing with HeLa cell standard, pH verification
Key Features	Instant RNase inactivation; standard for RNA extraction; compatible with organic and column purification; validated for tissues and cells

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For research use only, not for clinical use.