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| Product Name | Methanol, HPLC Grade, ≥99.9% |
| Catalog No. | ATR-SDS-0003 |
| Description | Ultra-high purity methanol with low UV absorbance and minimal particulate content, designed for use as a mobile phase component in HPLC and LC-MS analysis of biomolecules. |
| Intended Use | Mobile phase solvent for reversed-phase HPLC separation of peptides, proteins, and small molecules; protein precipitation agent in plasma sample preparation for LC-MS bioanalysis. |
| Principle / Technology | Methanol's moderate polarity and low viscosity make it an ideal organic modifier in reversed-phase chromatography; it effectively precipitates plasma proteins by disrupting their hydration shell. |
| Detection Method | GC purity, UV absorbance at 205–400 nm, fluorescence background |
| Sample Type | Peptide digests, protein preparations, plasma samples, pharmaceutical formulations |
| Performance Range / Specifications | Purity ≥99.9%; UV cutoff <205 nm; fluorescence background at 254 nm <1 ppb quinine sulfate equivalent |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | For protein precipitation: add 3 volumes of methanol to 1 volume plasma; vortex 30 sec; centrifuge at 14,000 × g 10 min; collect supernatant for analysis. |
| Components / Formulation | Methanol, filtered through 0.2 µm, HPLC tested |
| Storage Conditions | 15–30°C, flammable, tightly sealed |
| Shelf Life | 24 months after opening |
| Package Specifications | 1 L, 4 L |
| Product Form | Liquid |
| Quality Control | UV absorbance curve, fluorescence background, residue after evaporation <2 ppm, particulate per USP <788> |
| Key Features | Low UV cutoff for peptide detection; minimal background fluorescence; filtered for HPLC use; consistent batch quality |
For research use only, not for clinical use.
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