Dimethyl Sulfoxide (DMSO), Cell Culture Grade, ≥99.9%

Name: Dimethyl Sulfoxide (DMSO), Cell Culture Grade, ≥99.9%
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Cat.No: ATR-SDS-0001 Datasheet

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Product Name	Dimethyl Sulfoxide (DMSO), Cell Culture Grade, ≥99.9%
Catalog No.	ATR-SDS-0001
Description	Ultra-pure dimethyl sulfoxide tested for low cytotoxicity, suitable as a cryoprotectant for cell freezing and as a solvent vehicle for water-insoluble compounds in cell-based assays.
Intended Use	Cryopreservation of mammalian cell lines, dissolution of hydrophobic small molecule drugs and inhibitors for cell treatment studies, and solvent vehicle in MTT and other cytotoxicity assays.
Principle / Technology	DMSO penetrates cell membranes and depresses the freezing point of intracellular water, preventing ice crystal formation during cryopreservation; as a dipolar aprotic solvent, it solubilizes compounds with poor aqueous solubility.
Detection Method	Gas chromatography purity analysis, cell viability testing after DMSO exposure
Sample Type	Mammalian cells for cryopreservation, hydrophobic drug compounds, fluorescent dyes
Performance Range / Specifications	Purity ≥99.9% by GC; endotoxin <0.05 EU/mL; water content <0.1%
Sensitivity / LOD	N/A
Specificity	N/A
Reaction Conditions / Protocol	For cryopreservation: add to freezing medium at 5–10% v/v final concentration; for drug dissolution: prepare 100–1000x stock and dilute into culture medium keeping final DMSO <0.1% v/v.
Components / Formulation	Dimethyl sulfoxide, anhydrous, filtered through 0.1 µm membrane
Storage Conditions	15–30°C, protect from moisture, store under inert gas after opening
Shelf Life	36 months in unopened container
Package Specifications	50 mL, 100 mL, 500 mL
Product Form	Liquid, sterile-filtered
Quality Control	GC purity, water content by Karl Fischer titration, endotoxin, cell toxicity validation at working concentrations
Key Features	Cell culture tested; endotoxin free; sterile; anhydrous; validated for cryopreservation and drug dissolution

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For research use only, not for clinical use.