Alkaline Lysis Solution I, Plasmid Extraction, 50 mM Glucose, 25 mM Tris, 10 mM EDTA

Name: Alkaline Lysis Solution I, Plasmid Extraction, 50 mM Glucose, 25 mM Tris, 10 mM EDTA
Price: Contact Alta DiagnoTech for price USD
Availability: InStock

Cat.No: ATR-SDS-0012 Datasheet

Quantities:

- +

Add To Cart

Product Details Related Products

Product Name	Alkaline Lysis Solution I, Plasmid Extraction, 50 mM Glucose, 25 mM Tris, 10 mM EDTA
Catalog No.	ATR-SDS-0012
Description	The resuspension buffer (Solution I) of the classic alkaline lysis plasmid miniprep protocol, containing glucose for osmotic stabilization, Tris for pH buffering, and EDTA for divalent cation chelation.
Intended Use	Resuspension of pelleted bacterial cells as the first step in alkaline lysis plasmid DNA purification prior to addition of SDS-NaOH lysis solution.
Principle / Technology	Glucose maintains osmotic pressure to prevent premature cell lysis; Tris maintains pH 8.0 ensuring EDTA chelation of Mg2+ ions; EDTA destabilizes the outer membrane and inhibits endogenous nucleases.
Detection Method	Plasmid yield and purity by spectrophotometry, restriction enzyme digest verification, agarose gel analysis
Sample Type	Escherichia coli bacterial pellets from overnight culture
Performance Range / Specifications	Typical plasmid yield 20–50 µg per 5 mL overnight culture (high-copy plasmid)
Sensitivity / LOD	N/A
Specificity	N/A
Reaction Conditions / Protocol	Resuspend bacterial pellet in 250 µL (per 5 mL culture) by vortexing or pipetting; proceed immediately to Solution II addition.
Components / Formulation	Glucose 50 mM, Tris-HCl pH 8.0 25 mM, EDTA 10 mM, RNase A 100 µg/mL (optional)
Storage Conditions	2–8°C (when RNase added); 15–30°C (without RNase)
Shelf Life	6 months (with RNase); 24 months (without RNase)
Package Specifications	100 mL, 500 mL
Product Form	Liquid, ready to use
Quality Control	pH, osmolality, DNase/RNase free, plasmid extraction efficiency validation
Key Features	Ready to use; RNase A pre-added option; standard alkaline lysis component; validated for high and low copy plasmids

Cat.No	Product Name	Price
ATR-SDS-0024	Dimethylformamide, Anhydrous, ≥99.8%, ACS Grade	Add To Cart
ATR-SDS-0014	Ultra-Pure DMSO for Cell Biology (≥99.9%, Hybri-Max Equivalent)	Add To Cart
ATR-SDS-0019	Triton X-100, 10% Aqueous Solution, Proteomics Grade	Add To Cart
ATR-SDS-0011	Phenol-Chloroform-Isoamyl Alcohol (PCI), 25:24:1, pH 8.0	Add To Cart
ATR-SDS-0028	Urea, 8 M Solution, Deionized, for Protein Solubilization	Add To Cart
ATR-SDS-0029	Guanidine Hydrochloride, 6 M Solution, Molecular Biology Grade	Add To Cart
ATR-SDS-0006	Sodium Hydroxide Solution, 1 N, for pH Adjustment	Add To Cart
ATR-SDS-0015	Phenol:Chloroform:Isoamyl Alcohol 25:24:1, DNA/RNA Purification Grade	Add To Cart
ATR-SDS-0023	Urea, 8 M Solution, Molecular Biology Grade, Filtered	Add To Cart
ATR-SDS-0007	Glacial Acetic Acid, ACS Grade, ≥99.7%	Add To Cart

For research use only, not for clinical use.