Chloroform-Isoamyl Alcohol 24:1 Mixture, Molecular Biology Grade

Name: Chloroform-Isoamyl Alcohol 24:1 Mixture, Molecular Biology Grade
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Cat.No: ATR-SDS-0010 Datasheet

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Product Name	Chloroform-Isoamyl Alcohol 24:1 Mixture, Molecular Biology Grade
Catalog No.	ATR-SDS-0010
Description	A 24:1 (v/v) mixture of chloroform and isoamyl alcohol for the organic extraction phase in phenol-chloroform nucleic acid purification protocols, with isoamyl alcohol serving as an anti-foaming agent.
Intended Use	Organic phase component in phenol-chloroform extraction of nucleic acids; removal of proteins and lipids from DNA and RNA aqueous solutions during purification from biological samples.
Principle / Technology	Chloroform denatures proteins and dissolves lipids, partitioning them into the organic phase; isoamyl alcohol suppresses foam formation at the aqueous-organic interface, facilitating clean phase separation.
Detection Method	A260/A280 and A260/A230 purity ratios of extracted nucleic acids, agarose gel integrity verification
Sample Type	Cell lysates, tissue homogenates, bacterial suspensions, biological fluids
Performance Range / Specifications	A260/A280 ratio 1.8–2.0 for DNA; A260/A230 >2.0; protein contamination <1%
Sensitivity / LOD	N/A
Specificity	N/A
Reaction Conditions / Protocol	Add equal volume of PCI mixture, vortex, centrifuge at 12,000 × g for 5 min; transfer aqueous phase; repeat extraction if needed; precipitate nucleic acids from aqueous phase.
Components / Formulation	Chloroform and isoamyl alcohol in 24:1 volumetric ratio, DNase/RNase free
Storage Conditions	15–30°C, protect from light, tightly sealed to prevent evaporation
Shelf Life	24 months
Package Specifications	100 mL, 500 mL
Product Form	Liquid
Quality Control	Ratio verification, DNase/RNase free testing, extraction efficiency with standard DNA sample
Key Features	Premixed for convenience; nuclease free; anti-foaming; standard for phenol-chloroform extraction; consistent ratio

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For research use only, not for clinical use.