Primers & Probes

Molecular diagnostics are essential for detecting genetic disorders, infectious diseases, and neoplasms. The most frequently used technologies include PCR, biochips, FISH, DNA sequencing, mutation detection, and mass spectrometry. In molecular diagnostics, raw materials such as primers and probes are essential.

To fulfill client demands for increased volume and high-throughput synthesis of probes and primers, Alta DiagnoTech developed specialized automated systems for production and quality control. At the moment, the primers and probes produced by us are used extensively by many manufacturers of molecular diagnostic kits.

Overview of Primers and Probes

The amplification and analysis of specific genetic material is made possible through the molecular diagnostics of certain genes using primers and probes due to their functions as signaling molecules.

Primers:

Primers are single stranded fragments of DNA which are around 20 nucleotides long and are designed to complement specific regions of longer DNA or RNA molecules.
They initiate the synthesis of new DNA strands by DNA polymerase during PCR and similar techniques.
Primers are important for selective amplification of the target diagnostic areas and ensures the replicade genetic material is specific.

Probes:

Probes are short sequences of nucleotides which are usually labeled with fluorescent markers.
Probes are meant to bind with the sequence to which they are meant to attach, which is the one being amplified.
Probes, once bound to the target can emit signals like fluorescence which can be measured so that amplified genetic material can be detected and quantified.

Customization Solutions for Primers and Probes

We provide a comprehensive selection of probes and primers related to in vitro diagnostics (IVD) for clinical laboratories, especially in the emerging field of molecular diagnostics technology including, but not limited to:

Primers

Conventional PCR/qPCR Primers: Primer dimers and non-specific amplification is avoided through strict process control.

Multiplex PCR Primer Sets: Ensures simultaneous detection of multiple targets with compatibility optimization.

Highly Specific Primers: Minimized cross-reactions designed for SNP analysis, pathogen typing and other diagnostics.

Long-fragment PCR Primers: Used for large target fragments, like in NGS library preparation.

Modified Primers: For downstream applications, sequencing or cloning, these are modified by 5' fluorescent labeling, biotinylation, or phosphorylation.

Parameter	Details
Length	10–60 bases
Purification	Standard desalt or HPLC

Probes

TaqMan Probes: Fluorescently labeled with FAM, HEX, CY5, etc., paired with quenching groups (e.g., BHQ, TAMRA), suitable for qPCR.

Molecular Beacons: Detection of SNPs and low abundant targets with stem-loop structures of high specificity.

Hybridization Probes: FRET technology-dependent probes used for melting curve analysis.

MGB Probes: Detection sensitivity is enhanced with MGB probes due to incorporation of microgroove binding groups that strengthen the Tm value of short probes.

LNA-modified Probes: Designed for complex targets such as those with high GC content or mutation hotspots to increase hybridization stability.

Types	Details
Length	10–60 bases
Fluorophores	FAM; HEX; TET; Cy3; Cy5; Cy5.5; BODIPY; MGB; JOE; ROX
Quenchers	TAMRA; BHQ; ZEN; Eclipse; MGB
Purification	Standard desalt or HPLC

Advantages

High-Quality: We provide high-quality raw materials that are precisely matched to diagnostic applications such as qPCR and NGS.
Clean Workshops: High cleanliness level production workshops with high GMP Grade certification.
Fast Delivery: Quick, cost-effective delivery.
Comprehensive Support: Satisfy various molecular diagnostic reagent development steps in terms of primer and probe raw materials specifications and utilization.

Alta DiagnoTech's primer and probe products are widely applicable in the detection of infectious diseases, tumor screening, and hereditary disease diagnosis due to their remarkable sensitivity and specificity and excellent reproducibility across different batches. For any inquiries, don't hesitate to contact us.