Fluorescence Immunoassay (FIA)

Fluorescence immunoassay (FIA) is an analysis technique based on the specific recognition of antibodies-antigens to detect and quantify mobile target molecules by fluorescent signals. Alta DiagnoTech has mastered the advanced FIA technology and provides a wide range of FIA products that can be used for in vitro diagnostics (IVD) to accelerate the application of FIA technology in the IVD field.

What is FIA?

FIA is a quantitative method using fluorescently labeled antibodies or antigens to detect target substances in liquids like serum or urine, commonly used in clinical and biochemical diagnostics. It measures antigens and antibodies in liquids and tissue sections. Once a tracer binds to the antigen or antibody, a fluorescence detector observes or measures the fluorescence, determining presence, localization, and quantifying levels.

Fig.1 Principle of fluorescence immunoassay. (Wang, et al., 2021)

Applications of FIA Products in IVD

The wide applicability and value of FIA in IVD help to provide rapid, sensitive, and reliable diagnostic information to support clinical decision-making. The following are some typical applications of FIA products in IVD:

Alta DiagnoTech provides advanced, precise FIA products that help clients improve the accuracy and efficiency of their clinical diagnostics.

Please click on the filter box below to quickly locate the IVD products you need.

Enzyme-Linked Fluorescent Assay Test Kits

Fluorescent Immunochromatography Kits

Flow Cytometry Kits (FIA)

FIA Microscopy Kits

Indirect FIA Kits

Enzyme-Linked Fluorescent Assay Test Kits

Enzyme-linked fluorescent assay (ELFA) kits combine the high specificity of enzyme-linked immunosorbent assay (ELISA) with the ultra-sensitivity of fluorescence detection, enabling precise quantification of target analytes across a wide dynamic range for diverse biomedical diagnostic applications.

Features

• Ultra-high Sensitivity: ELFA sensitivity surpasses spectrophotometry by 2-3 orders of magnitude, enabling precise detection of picogram-level (pg/mL) trace substances.
• Wide Linear Range: ELFA fluorescence signals exhibit strong linear correlation with concentration, covering a dynamic range of 3-4 orders of magnitude without requiring repeated sample dilutions.
• Multi-Target Compatibility: ELFA supports diverse target detection, including proteins, hormones, drugs, and pathogens, fully meeting in vitro diagnostic (IVD) requirements.

Common ELFA Kits

• Hormone Detection Kits
• Infectious Disease Detection Kits

• Tumor Marker Detection Kits
• Drug Concentration Detection Kits

Product List

Applications

• Infectious Diseases Diagnosis: ELFA kits enable rapid detection of pathogen-specific antigens or antibodies, such as viral markers for HIV and HCV, supporting early diagnosis and infection control.
• Chronic Disease Management: Through quantitative analysis of hormones or metabolic markers, ELFA kits facilitate long-term monitoring and treatment plan adjustments for chronic conditions like diabetes and thyroid disorders.
• Drug Concentration Monitoring: ELFA kits quantitatively measure blood concentrations of immunosuppressants (e.g., tacrolimus), guiding personalized dosing for organ transplant patients to reduce rejection risks.

Fluorescent Immunochromatography Kits

Fluorescent immunochromatography kits utilize fluorescent microspheres as markers, combining the rapid separation characteristics of immunochromatography to achieve target detection through antigen-antibody specific binding.

Features

• Rapid and Convenient: Testing typically completes within 15 minutes without complex equipment, making it ideal for rapid on-site screening.
• High Specificity: Antigen-antibody specific binding minimizes cross-reaction risks, suitable for complex samples (e.g., whole blood, saliva).
• Multi-scenario Applicability: Supports both quantitative and qualitative detection, compatible with various sample types, including serum, plasma, and urine.

Common ELFA Kits

• Virus Typing Detection Kits
• Inflammation Marker Detection Kits

• Tumor Marker Detection Kits
• Drug Abuse Detection Kits

Product List

Applications

• Disease Diagnosis and Screening: Fluorescent immunochromatography kits can be used to detect infectious diseases, tumor markers, and endocrine hormones to assist in early diagnosis and prognosis monitoring.
• Drug Abuse Detection: Fluorescent immunochromatography kits can be used to detect illicit drugs and their metabolites in urine, blood, and other samples, providing rapid and accurate results for drug abuse screening.
• Reproductive Health Testing: The fluorescent immunoassay kit can be used for reproductive health testing, determining pregnancy status in women, and measuring sex hormones in both men and women to aid in disease diagnosis and evaluation.

Flow Cytometry Kits (FIA)

Utilizing flow cytometry and FIA technology, the kit simultaneously captures cell light scattering characteristics and target molecule expression signals, enabling high-throughput, multiparametric, and ultra-sensitive phenotypic profiling and functional monitoring.

Features

• Multi-parameter Detection: Enables simultaneous analysis of multiple targets on cell surfaces and within cells, achieving precise classification of complex phenotypes.
• High-throughput Analysis: Suitable for large-scale sample screening, significantly enhancing experimental efficiency.
• Wide-ranging Applications: Covers immunology, oncology, hematology, and other fields.

Common Flow Cytometry Kits (FIA)

• DNA Content & Apoptosis Flow Cytometry Kits
• Platelet Activation Flow Cytometry Kits

• Tumor Cell Flow Cytometry Kits
• Hematopoietic Stem Cell Enumeration Kits

Product List

Applications

• Immune Cell Subtype and Functional Assessment: Flow cytometry kits (FIA) utilize multicolor fluorescently labeled antibodies to precisely analyze the proportion and activation status of T cells, B cells, NK cells, and other subsets, thereby aiding in leukemia/lymphoma classification and immunodeficiency disease diagnosis.
• Cell Cycle and Apoptosis Analysis: Combining DNA staining with apoptosis markers, flow cytometry kits (FIA) enable quantitative assessment of cellular proliferation activity and chemotherapy-induced apoptosis rates, guiding optimization of tumor treatment regimens.
• Platelet Function Testing: By labeling platelet activation markers, flow cytometry kits (FIA) can assess thrombotic risk and evaluate antiplatelet drug efficacy, making them suitable for diagnosing cardiovascular and hemorrhagic disorders.

FIA Microscopy Kits

FIA microscopy kits for IVD typically operate on the principle of antibody-antigen specific binding, utilizing fluorescent labeling technology to achieve highly sensitive detection of target molecules such as proteins, viruses, and bacteria. Their core advantage lies in combining the specificity of immune reactions with the sensitivity of fluorescence technology, enabling rapid and precise quantitative or qualitative analysis of trace biomarkers within samples.

Features

• High Sensitivity: Detects target molecules down to picogram levels (pg/mL) through fluorophore labeling and time-resolved technology.
• Rapid Detection: Most kits complete testing within a short timeframe, meeting on-site screening requirements.
• Flexible Analysis: Enables qualitative localization or quantitative concentration analysis of target molecules using equipment such as fluorescence microscopes, flow cytometers, or time-resolved fluorescence instruments.

Common Flow Cytometry Kits (FIA)

• Quantum Dot Immunofluorescence Kits
• Fluorescein-labeled Kits

• Stem Cell Marker Detection Kits
• Pathogen Detection Kits

Product List

Applications

• Rapid Infectious Disease Testing: The kit enables rapid qualitative detection of nucleoprotein antigens from pathogens such as respiratory syncytial virus (RSV) and influenza virus, aiding in infectious disease diagnosis.
• Allergy and Immunological Disease Diagnosis: The kit measures total serum IgE levels to assist in assessing allergy severity.
• Metabolic Disease Analysis: The kits can be used for quantitative detection of hormones or metabolites, aiding in the diagnosis of endocrine and metabolic disorders such as thyroid dysfunction and diabetes.

FIA Microscopy Kits

Indirect FIA kits utilize antigen-antibody specific binding principles to achieve qualitative/quantitative detection of target molecules. These kits are widely applied in infectious disease diagnostics, autoimmune disease research, and tumor marker monitoring. They support high-throughput testing, enabling simultaneous analysis of multiple antigens to significantly enhance diagnostic efficiency.

Features

• Multicolor Fluorescent Labeling: Enables simultaneous detection of multiple targets, facilitating multi-marker colocalization and high-throughput analysis in complex samples.
• Broad Sample Compatibility: Suitable for diverse sample types, including serum, tissue, and cell lysates, offering flexibility across various testing scenarios.
• Rapid Diagnostic Efficiency: Integrating biochip technology allows simultaneous analysis of multiple antigens, significantly reducing detection time and enhancing throughput.

Common Indirect FIA Kits

• Antinuclear Antibody (ANA) FIA Kits
• dsDNA Antibody FIA Kits

• HIV Antibody FIA Kits
• SARS-CoV-2 Antigen FIA Kits

Product List

Applications

• Precise Classification of Autoimmune Diseases: The kit enables efficient screening for autoimmune diseases such as systemic lupus erythematosus, providing reliable disease classification and diagnostic support for clinical practice.
• Rapid Detection of Respiratory Viruses: Within a short timeframe, the kit identifies pathogens like the respiratory syncytial virus, aiding in the treatment of respiratory diseases.
• Simultaneous Multi-Allergen Screening: This kit enables one-step detection of multiple allergen-specific IgE antibodies in serum, achieving precise allergen localization and graded assessment.

Alta DiagnoTech's innovative FIA products deliver unprecedented precision and agility in IVD. Our FIA products capture every microscopic change, enabling efficient decision-making and patient care breakthroughs. Together, let's open up new horizons in health diagnostics, and feel free to contact us to explore the potential possibilities.