In Situ Hybridization (ISH)

In situ hybridization (ISH) kits are tools for the detection of specific nucleic acid sequences, which help identify molecular features of diseases and aid in pathological diagnosis by providing accurate gene and chromosome localization information. Based on a diversified portfolio of IVD products and cutting-edge diagnostic technologies, Alta DiagnoTech offers several types of ISH kits, such as CISH, FISH, SISH, and RNA ISH kits, to meet the challenges of complexity and precision in clinical diagnostics.

Overview of ISH

ISH is a commonly used laboratory technique for analyzing gene expression and the localization of specific DNA and RNA molecules in cells and is an important tool for basic science research and clinical diagnosis. According to the type of method for in situ RNA and DNA target display, it can be divided into fluorescence in situ hybridization (FISH), chromogenic in situ hybridization (CISH) and silver-enhanced in situ hybridization (SISH).

Fig.1 Detection of RNA in breast cancer cells using ISH. (Veselinyová, et al., 2021)

Applications of ISH Products in IVD

With the advancement of technology, ISH products have been continuously developed and optimized, and they play an important role in the field of IVD, such as cancer diagnosis, genetic disease screening, and infectious disease detection, providing important support for precision medicine and personalized treatment.

Please click on the filter box below to quickly locate the IVD products you need.

FISH Test Kits

CISH Test Kits

RNA ISH Test Kits

Quantitative ISH Test Kits

FISH Test Kits

Fluorescence in situ hybridization (FISH) kits are highly specific molecular cytogenetic testing tools that use fluorescently labeled nucleic acid probes to accurately locate target DNA/RNA sequences. They are widely used in the diagnosis of genetic diseases, tumors, and prenatal conditions. Alta DiagnoTech provides high-quality FISH test kits to support precision medicine and disease diagnosis.

Key Points of FISH Technology

• Specimen Selection: Mitotic cell chromosomes or interphase cells.
• Labeling Probes: Biotin, digoxigenin, dinitrophenyl (DNP), amino acetyl fluorine (AAF), etc.

Applications

Product List

CISH Test Kits

As a leading supplier of in vitro diagnostic (IVD) products, Alta DiagnoTech offers colorimetric in situ hybridization (CISH) test kits with intuitive result interpretation, making gene status interpretation simpler and more reliable.

Introduction to CISH Technology

CISH is a process that uses labeled complementary DNA or RNA chains to locate specific nucleic acid sequences in tissue specimens. It can be used to evaluate gene amplification, gene deletion, chromosome translocation, and chromosome number abnormalities.

Features:

• Observe tissue morphology while assessing gene status.
• Use bright field microscopy.
• The processing method is similar to immunohistochemistry.
• Quantitative results.

Product List

RNA ISH Test Kits

RNA ISH test kits are used for the specific detection and localization of specific RNA molecules in cells or tissue sections, and are widely used in pathological diagnosis and disease research. Alta DiagnoTech supplies high-quality and stable RNA ISH test kits and is a trusted partner in in vitro diagnostics (IVD).

RNA ISH Technology

RNA ISH technology detects the expression information of target RNA in tissues or cells through complementary binding of probes. It provides a new perspective on nucleic acid visualization, filling the gap in RNA visualization and quantification under a microscope.

Product List

Applications

• Companion Diagnostics: Detect specific genes in tumor tissue to provide a basis for matching targeted drugs.

• Prognosis Assessment: Detect biomarkers associated with disease aggressiveness to assist in prognosis assessment.

• Pathogen Test: Specifically identify viral infections, determine the location of pathogens, and identify active infections.

Quantitative ISH Test Kits

Alta DiagnoTech provides high-precision quantitative in situ hybridization (ISH) test kits to help clients achieve molecular-level detection and analysis.

What are Quantitative ISH Test Kits?

Quantitative ISH Test Kits combine the precise localization advantages of ISH technology with quantitative analysis capabilities. ISH technology uses labeled nucleic acid probes to specifically bind to target nucleic acid sequences in samples, enabling in situ detection of specific genes or nucleic acid sequences in cells or tissues. The quantitative function allows for precise measurement of the quantity or expression level of target nucleic acids.

Product List

Applications

Genetic Disease Test

Detects gene mutations, deletions, or amplifications to aid in the diagnosis of genetic diseases.

Tumor Diagnosis

Quantitatively analyzes tumor marker genes to assist in determining tumor type, staging, and efficacy evaluation.

Pathogen Test

Locates and quantitatively detects pathogen nucleic acids for the diagnosis of infectious diseases.

Alta DiagnoTech offers a line of ISH kits that provide efficient and accurate assays for modern scientific research and IVD. Regardless of your research direction, we offer a tailor-made product portfolio to help you succeed in diverse applications. Please feel free to contact us, we are happy to provide you with more information and support.