TRFIA

Time-resolved fluorescence immunoassay (TRFIA) is a highly sensitive and specific analysis technique for the quantitative determination of target substances which is widely used in biomedical research and clinical diagnostics. Alta DiagnoTech is dedicated to the field of in vitro diagnostics (IVD) and clinical diagnostics, with a team of experienced researchers and leading experts in the industry, to provide advanced TRFIA products to clients.

Introduction to TRFIA

TRFIA is a highly sensitive immunoassay technique widely used in clinical diagnostics and biological research. TRFIA utilizes fluorescent markers for detection, the most commonly used of which are chelates formed by lanthanide elements (e.g., europium, terbium, samarium, and gadolinium), which have unique long-lived fluorescent properties. TRFIA technology enables the simultaneous quantification of multiple analytes over a wide dynamic range and is suitable for the analysis of samples at different concentrations.

Fig.1 Schematic diagram of the TRFIA detection principle and steps. (Li, et al., 2024)

Applications of TRFIA Products in IVD

IVD products developed based on TRFIA technology have the advantage of high sensitivity and low background interference, which makes them suitable for in vitro/clinical diagnostic applications such as infectious disease detection, cardiovascular disease marker detection, and endocrine disruption detection.

Alta DiagnoTech specializes in providing high-quality TRFIA products with an advanced technical team and innovative research and development capabilities. Our TRFIA products are known for their high sensitivity and specificity, enabling precise biomarker detection in clinical and research applications.

Please click on the filter box below to quickly locate the TRFIA products you need.

FIA Kits (TRFIA)

TRIFA Kits

Flow Cytometry Kits (TRFIA)

FIA Kits (TRFIA)

Fluorescent immunoassay (FIA) is a highly sensitive detection technology based on antigen-antibody specific binding reactions, enabling quantitative analysis of target molecules through fluorescent labels such as quantum dots and rare-earth fluorescent microspheres. Alta DiagnoTech offers high-performance FIA kits (TRFIA) covering applications including infectious disease detection, chronic disease management, and early tumor screening.

Product List

Features

• High Sensitivity: Fluorescent labeling technology enables detection limits as low as the pg/mL range, far surpassing traditional methods.
• Multi-marker Simultaneous Testing: Supports multi-channel fluorescent labeling for concurrent detection of multiple targets.
• Simple Operation: Requires no complex pretreatment; samples can be added directly, making it suitable for primary care and point-of-care testing.
• Reliable Results: Built-in control lines and detection lines ensure result accuracy.

TRIFA Kits

TRFIA is a highly sensitive immunological analysis technique based on lanthanide-labeled reagents, enabling time-resolved measurements through its long fluorescence lifetime characteristics. It is widely employed for the quantitative detection of tumor markers, hormones, and viral antigens. Alta DiagnoTech offers multiple TRFIA kits covering disease diagnostics and hormone analysis applications.

Product List

Features

• Ultra-high Sensitivity: Lanthanide-labeled detection achieves limits as low as the pg level, enabling precise quantification of low-abundance substances.
• Strong Anti-interference Capability: Time-resolved technology combined with long fluorescence lifetimes effectively eliminates sample matrix and non-specific fluorescence interference, delivering stable and reliable results.
• Wide Detection Range: The calibration curve spans multiple orders of magnitude, enabling simultaneous detection of high- and low-concentration samples. This reduces dilution steps and enhances testing efficiency.
• Flexible Sample Types: Supports direct testing of various samples, including serum, whole blood, and saliva, without complex pretreatment.

Flow Cytometry Kits (TRFIA)

Flow cytometry is a highly efficient single-cell analysis technique based on fluorescent labeling technology, enabling simultaneous analysis of cell size, granularity, and surface/intracellular markers. The flow cytometry kits provided by Alta DiagnoTech are specifically designed for IVD, utilizing high-affinity fluorescently labeled antibodies. They are suitable for applications such as preclinical pharmacodynamic evaluation, immune monitoring, and hematological disease diagnosis.

Product List

Features

• Ultra-Sensitive Multicolor Detection: Combining flow cytometry with TRFIA technology enables target detection down to the pg level.
• Strong Anti-Interference Capability: Utilizes long fluorescence lifetime and time-delayed measurement techniques to effectively eliminate sample autofluorescence and instrument noise interference, ensuring signal specificity.
• High-Throughput Detection: Compatible with automated flow cytometry platforms for multi-well plates, processing hundreds of samples per batch to support large-scale screening.
• Multi-Scenario Application Coverage: Covers immune cell typing, cell function testing, and pathogen screening, meeting diagnostic needs for oncology, infectious diseases, and autoimmune disorders.

Alta DiagnoTech is committed to providing high-quality TRFIA IVD products with an exceptional team and leading-edge technology to help clients with novel solutions in rapid, accurate, and sensitive disease detection. We listen attentively to our client's needs to ensure a customized product portfolio for each client. Please feel free to contact us for further support and services.