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| Product Name | Acetonitrile, HPLC Gradient Grade, ≥99.9% |
| Catalog No. | ATR-SDS-0009 |
| Description | Ultra-high purity acetonitrile with minimal UV absorbance and gradient baseline stability, the preferred organic solvent for reversed-phase HPLC and LC-MS analysis of biomolecules. |
| Intended Use | Mobile phase organic component for peptide mapping, protein analysis, and small molecule quantification by HPLC and UHPLC; solvent for solid-phase extraction of biological samples. |
| Principle / Technology | Acetonitrile's strong elution strength, low viscosity, and low UV cutoff make it the optimal organic modifier for separating peptides and proteins on C18 columns; its volatility enhances electrospray ionization efficiency. |
| Detection Method | GC purity, UV absorbance at 200–400 nm, gradient baseline drift, fluorescence background |
| Sample Type | Peptide digests, protein preparations, small molecule drugs, biological extracts |
| Performance Range / Specifications | Purity ≥99.9%; UV cutoff <190 nm; gradient baseline drift <0.001 AU at 210 nm |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Prepare binary gradient with 0.1% TFA or formic acid in water; typical gradient 5–95% acetonitrile over 30–120 minutes. |
| Components / Formulation | Acetonitrile, HPLC gradient grade, 0.2 µm filtered |
| Storage Conditions | 15–30°C, flammable, tightly sealed |
| Shelf Life | 24 months |
| Package Specifications | 1 L, 4 L |
| Product Form | Liquid |
| Quality Control | GC purity, UV absorbance curve, fluorescence, gradient baseline, residue after evaporation, water content |
| Key Features | HPLC gradient tested; low UV absorbance; minimal baseline drift; consistent batch quality; suitable for UHPLC |
For research use only, not for clinical use.
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