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| Product Name | Tween-20, 10% Aqueous Solution, Molecular Biology Grade |
| Catalog No. | ATR-SDS-0020 |
| Description | A 10% (w/v) aqueous solution of polysorbate 20 (Tween-20), a non-ionic detergent widely used in immunoassays, Western blotting, and ELISA procedures as a blocking agent and wash buffer additive. The pre-diluted format ensures reproducible surfactant concentrations while avoiding the difficult pipetting of pure viscous Tween-20. Each lot is tested for peroxidase activity and peroxide content to ensure compatibility with HRP-based detection systems. |
| Intended Use | ELISA wash buffer component; Western blot membrane washing and blocking; immunohistochemistry wash buffer; surface passivation for microfluidic devices; protein stabilization in dilute solutions; reduction of non-specific binding in immunoassays. |
| Principle / Technology | Non-ionic surfactant that reduces hydrophobic interactions between proteins and surfaces, minimizing non-specific binding in immunoassay workflows |
| Detection Method | Typical working concentration: 0.05–0.5% (v/v); add to PBS or TBS to prepare PBST or TBST wash buffers |
| Components / Formulation | Polysorbate 20 (Tween-20, CAS 9005-64-5), 10% (w/v) in ultrapure water; filtered through 0.22 µm |
| Storage Conditions | Store at 15–25 °C; protect from light; keep container tightly closed |
| Shelf Life | 24 months from manufacture date; use within 12 months after opening |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles |
| Product Form | Clear, slightly viscous, colorless to pale yellow liquid |
| Key Features | Molecular biology grade; tested peroxidase-free; peroxide <0.002%; pre-filtered 0.22 µm; eliminates pipetting errors from viscous pure detergent; consistent 10.0 ± 0.2% (w/v) concentration; low aldehyde content for sensitive detection applications |
| Purity | Polysorbate 20 10.0 ± 0.2% (w/v); water content approximately 90% |
| Concentration | 10% (w/v) aqueous solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | pH 6.0–7.5 (neat solution at 25 °C) |
| Shipping Conditions | Ambient temperature; non-hazardous; protect from freezing |
| Expiration Date / Stability | 24 months unopened; 12 months after opening; discard if solution becomes turbid or develops rancid odor indicating degradation |
| Regulatory / Compliance | For laboratory and research use only; CAS 9005-64-5; not classified as dangerous goods; food-grade polysorbate compliant with FCC/USP-NF monographs |
| Compatibility | Compatible with standard laboratory materials; avoid prolonged storage in polystyrene containers which may leach inhibitors; use polypropylene or glass for long-term storage |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For ELISA wash buffer, add to PBS to 0.05% (v/v) final (PBST). For Western blot blocking, 0.1% Tween-20 in TBS (TBST) with 5% non-fat dry milk or BSA. For membrane stripping, increase Tween-20 to 0.5% in stripping buffer. Always prepare wash buffers fresh or store at 4 °C for up to 1 week. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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