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| Product Name | Triton X-100, 10% Aqueous Solution, Proteomics Grade |
| Catalog No. | ATR-SDS-0019 |
| Description | A 10% (w/v) aqueous solution of Triton X-100 (octylphenol ethoxylate), a non-ionic surfactant used extensively for cell lysis, membrane protein solubilization, and permeabilization in biochemical and cell biology applications. This pre-diluted solution eliminates the handling challenges of the viscous pure detergent, providing accurate and reproducible detergent concentrations for standardized protocols. Proteomics grade certification ensures low carbonyl and peroxide levels that could interfere with protein analysis. |
| Intended Use | Cell membrane permeabilization for immunofluorescence and intracellular staining; protein extraction from mammalian cells and tissues; membrane protein solubilization; ELISA wash buffer additive; nuclear envelope permeabilization. |
| Principle / Technology | Non-ionic detergent that disrupts lipid-lipid and lipid-protein interactions in biological membranes without denaturing proteins |
| Detection Method | Typical working concentration: 0.1–1.0% (v/v); dilute stock solution with appropriate buffer immediately before use |
| Components / Formulation | Triton X-100 (CAS 9002-93-1), 10% (w/v) in ultrapure water; filtered through 0.22 µm membrane |
| Storage Conditions | Store at 15–25 °C; protect from light; keep container tightly closed |
| Shelf Life | 24 months from manufacture date; use within 12 months after opening |
| Package Specifications | 100 mL, 500 mL, 1 L HDPE bottles; amber bottle option for light-sensitive applications |
| Product Form | Clear, slightly viscous, colorless to pale yellow liquid |
| Key Features | Proteomics grade: peroxide <0.005%, carbonyl <0.01%; pre-filtered 0.22 µm; eliminates weighing and dissolution of viscous pure detergent; consistent 10.0 ± 0.2% (w/v) concentration lot-to-lot; tested negative for protease activity |
| Purity | Triton X-100 10.0 ± 0.2% (w/v); water content 90.0 ± 0.2% |
| Concentration | 10% (w/v) aqueous solution; 10 g Triton X-100 per 100 mL solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | pH 6.5–7.5 (neat solution at 25 °C) |
| Shipping Conditions | Ambient temperature; non-hazardous for transport; protect from freezing |
| Expiration Date / Stability | 24 months unopened; 12 months after opening; discard if solution develops strong yellow color or precipitate |
| Regulatory / Compliance | For laboratory and research use only; CAS 9002-93-1; not classified as dangerous goods; REACH registered substance |
| Compatibility | Compatible with polypropylene, polyethylene, glass, and stainless steel; avoid prolonged contact with polystyrene which may be softened; avoid polycarbonate |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For cell permeabilization in immunofluorescence, use 0.1–0.5% Triton X-100 in PBS for 5–15 minutes at room temperature. For protein extraction, use 1% Triton X-100 in appropriate lysis buffer with protease inhibitors. Triton X-100 absorbs at 280 nm; use a detergent-compatible protein assay (BCA or Bradford) for quantification. Protect from light during long-term storage. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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