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| Product Name | Phenol:Chloroform:Isoamyl Alcohol 25:24:1, DNA/RNA Purification Grade |
| Catalog No. | ATR-SDS-0015 |
| Description | A pre-mixed phenol-chloroform-isoamyl alcohol solution at 25:24:1 (v/v) ratio, saturated with Tris buffer at pH 8.0, ready for immediate use in nucleic acid purification. The phenol denatures proteins and strips them from nucleic acid, chloroform facilitates phase separation and removes lipids, and isoamyl alcohol reduces foaming during extraction. This solution is widely used for removing proteins from DNA and RNA preparations following enzymatic reactions. |
| Intended Use | Protein removal from nucleic acid solutions by organic phase extraction in molecular biology workflows. |
| Principle / Technology | Phenol-mediated protein denaturation with chloroform-enhanced phase separation |
| Detection Method | Spectrophotometric A260/A280 ratio assessment of purified nucleic acid |
| Sample Type | DNA and RNA solutions after restriction digestion, PCR, reverse transcription, or in vitro transcription |
| Performance Range / Specifications | Volume ratio: 1 volume PCI to 1 volume aqueous sample; phase separation by centrifugation at 12,000–16,000 × g |
| Sensitivity / LOD | Protein removal efficiency: ≥99.9% as assessed by residual protein assay on extracted aqueous phase |
| Specificity | Non-selective protein precipitation; effective across all protein types and sizes |
| Reaction Conditions / Protocol | Add equal volume of PCI to aqueous sample; vortex 15 sec; centrifuge 5 min at room temperature; carefully transfer upper aqueous phase to fresh tube |
| Components / Formulation | Phenol (Tris-buffered, pH 8.0): chloroform: isoamyl alcohol (25:24:1 v/v), containing 0.1% 8-hydroxyquinoline as antioxidant |
| Storage Conditions | 2–8 °C, protected from light; 8-hydroxyquinoline imparts yellow color; discard if color turns pink indicating oxidation |
| Shelf Life | 12 months at 2–8 °C |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, organic phase, yellow-colored |
| Quality Control | Phase separation integrity verified; aqueous phase pH confirmed 7.8–8.2; DNA recovery from extraction ≥95% |
| Key Features | Pre-mixed and pre-equilibrated format eliminates hazardous manual mixing of individual organic solvents |
| Purity | ≥99% by GC or HPLC; suitable for molecular biology applications |
| Concentration | As specified; typically neat or concentrated solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | Not applicable — organic solvent |
| Shipping Conditions | Ambient temperature; flammable liquids shipped per dangerous goods regulations |
| Expiration Date / Stability | 24–36 months in sealed container under inert atmosphere |
| Regulatory / Compliance | Research use; hazardous material classification per GHS; SDS provided |
| Compatibility | Compatible with standard laboratory glassware and polypropylene vessels |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | Handle in fume hood; flammable; avoid contact with oxidizers; store away from ignition sources |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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