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| Product Name | NP-40, 10% Solution, Cell Lysis Grade, Non-Denaturing |
| Catalog No. | ATR-SDS-0021 |
| Description | A 10% (w/v) aqueous solution of Nonidet P-40 Substitute (IGEPAL CA-630 equivalent), a non-ionic, non-denaturing detergent for gentle cell lysis and protein extraction. This formulation preserves protein-protein interactions and enzymatic activity while effectively solubilizing cytoplasmic and membrane proteins. Ideal for immunoprecipitation, co-immunoprecipitation, and native protein complex analysis where denaturing detergents like SDS are unsuitable. |
| Intended Use | Gentle whole-cell lysis for immunoprecipitation and co-immunoprecipitation; cytoplasmic and nuclear protein extraction; membrane protein solubilization; organelle isolation; preparation of whole-cell lysates for Western blotting. |
| Principle / Technology | Non-ionic detergent that solubilizes cellular membranes through hydrophobic interaction with lipid bilayers while preserving native protein conformation and protein-protein interactions |
| Detection Method | Typical working concentration: 0.1–1.0% (v/v); add to lysis buffer (e.g., 50 mM Tris-HCl pH 7.4, 150 mM NaCl, protease inhibitors) immediately before use |
| Components / Formulation | Octylphenoxypolyethoxyethanol (IGEPAL CA-630, CAS 9002-93-1), 10% (w/v) in ultrapure water; 0.22 µm filtered |
| Storage Conditions | Store at 2–8 °C; protect from light; do not freeze |
| Shelf Life | 18 months from manufacture date; use within 6 months after opening |
| Package Specifications | 50 mL, 100 mL, 500 mL HDPE bottles |
| Product Form | Clear to pale yellow, moderately viscous liquid |
| Key Features | Non-denaturing; preserves protein-protein interactions; suitable for Co-IP and enzyme activity assays; 10% pre-diluted format for accurate pipetting; tested negative for protease, DNase, and RNase activity; low UV absorbance at 280 nm for protein monitoring |
| Purity | IGEPAL CA-630 10.0 ± 0.3% (w/v); peroxide <0.005% |
| Concentration | 10% (w/v) aqueous solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | pH 6.0–8.0 (neat solution) |
| Shipping Conditions | Cold pack 2–8 °C; short-term ambient transport <48 hours acceptable |
| Expiration Date / Stability | 18 months at 2–8 °C; 6 months after opening; discard if solution develops strong yellow or brown discoloration indicating oxidation |
| Regulatory / Compliance | For laboratory and research use only; CAS 9002-93-1; not classified as hazardous for transport; REACH registered |
| Compatibility | Compatible with standard laboratory plastics and glass; avoid polystyrene for long-term storage due to detergent absorption potential |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | For whole-cell lysis, use 0.5–1.0% NP-40 in lysis buffer for 30 minutes on ice with gentle agitation. For nuclear extract preparation, use 0.1% NP-40 for cytoplasmic membrane lysis followed by nuclear pellet extraction. NP-40 interferes with SDS-PAGE at concentrations >1%; dilute lysates accordingly before loading. Always include fresh protease and phosphatase inhibitors in lysis buffer. |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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