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| Product Name | Guanidine Hydrochloride, 8 M Solution, Molecular Biology Grade |
| Catalog No. | ATR-SDS-0016 |
| Description | An 8 M guanidine hydrochloride solution prepared with nuclease-free water and filtered for use in nucleic acid purification protocols. Guanidine hydrochloride is a chaotropic salt that denatures proteins including nucleases and facilitates the binding of nucleic acids to silica matrices in column- and bead-based purification systems. This ready-to-use solution eliminates the need for weighing and dissolving the hygroscopic solid, reducing preparation time and ensuring consistent concentration. |
| Intended Use | Chaotropic denaturant for protein inactivation and facilitation of nucleic acid binding to silica surfaces in purification workflows. |
| Principle / Technology | Protein denaturation by chaotropic disruption of hydrogen bonding and hydrophobic interactions |
| Detection Method | Nucleic acid recovery assessed by spectrophotometry and gel electrophoresis |
| Sample Type | Tissue, cell, and bacterial lysates for DNA or RNA extraction |
| Performance Range / Specifications | Working concentration range: 4–6 M for silica binding; 1–2 M for protein denaturation in enzymatic reactions |
| Sensitivity / LOD | Complete RNase inactivation within 30 sec at ≥4 M concentration at room temperature |
| Specificity | Denatures all protein classes; effective against RNases, DNases, and proteases equally |
| Reaction Conditions / Protocol | Add to cell or tissue lysate at appropriate ratio per extraction protocol; typical use: 1 volume lysate to 1 volume 8 M GuHCl |
| Components / Formulation | 8 M guanidine hydrochloride in nuclease-free water, pH 6.0–6.5, 0.22 µm filtered |
| Storage Conditions | Room temperature; guanidine hydrochloride may crystallize below 15 °C; warm to redissolve |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, clear to faint yellow |
| Quality Control | Chaotropic activity verified by RNase inactivation assay; DNase/RNase tested negative; endotoxin ≤0.1 EU/mL |
| Key Features | Ready-to-use 8 M solution eliminates hazards of weighing fine hygroscopic guanidine hydrochloride powder |
| Purity | ≥99% by GC or HPLC; suitable for molecular biology applications |
| Concentration | As specified; typically neat or concentrated solution |
| Activity / Unit Definition | Solubilization capacity as specified in product description |
| Molecular Weight | As specified in product documentation |
| Source / Origin | Synthetic, purified, and quality tested |
| pH Range / Optimal pH | Not applicable — organic solvent |
| Shipping Conditions | Ambient temperature; flammable liquids shipped per dangerous goods regulations |
| Expiration Date / Stability | 24–36 months in sealed container under inert atmosphere |
| Regulatory / Compliance | Research use; hazardous material classification per GHS; SDS provided |
| Compatibility | Compatible with standard laboratory glassware and polypropylene vessels |
| Recommended Buffer System | Use directly or dilute in compatible solvent as per protocol |
| Application Notes / Precautions | Handle in fume hood; flammable; avoid contact with oxidizers; store away from ignition sources |
| Batch-to-Batch Consistency | Purity within ±0.5% of specification; water content within tolerance |
For research use only, not for clinical use.
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