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| Product Name | Ethanol Absolute, Molecular Biology Grade, ≥99.5% |
| Catalog No. | ATR-SDS-0002 |
| Description | Highly purified absolute ethanol suitable for nucleic acid precipitation, sanitization of work surfaces in PCR laboratories, and as a solvent for molecular biology reagents. |
| Intended Use | Precipitation and washing of DNA and RNA during purification procedures, preparation of 70% ethanol for surface decontamination in nucleic acid workflows, and dissolution of molecular biology reagents. |
| Principle / Technology | Ethanol reduces the dielectric constant of aqueous solutions, promoting nucleic acid aggregation and precipitation in the presence of monovalent cations; it also denatures proteins and acts as a surface disinfectant. |
| Detection Method | GC purity analysis, DNase/RNase free certification |
| Sample Type | Purified nucleic acid solutions, molecular biology reagent preparations |
| Performance Range / Specifications | Purity ≥99.5%; DNase, RNase, and protease not detected |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | For DNA precipitation: add 2–2.5 volumes to sample with 0.3 M sodium acetate; incubate at -20°C; centrifuge; wash pellet with 70% ethanol. |
| Components / Formulation | Ethanol absolute, filtered, certified nuclease free |
| Storage Conditions | 15–30°C, flammable, tightly sealed |
| Shelf Life | 36 months |
| Package Specifications | 500 mL, 1 L, 4 L |
| Product Form | Liquid |
| Quality Control | Nuclease activity testing, GC purity, water content, UV absorbance at 260 and 280 nm |
| Key Features | Nuclease free; molecular biology grade; suitable for RNA work; UV-transparent; consistent lot quality |
For research use only, not for clinical use.
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