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| Product Name | Essential 8 Medium, for Human Pluripotent Stem Cell Culture |
| Catalog No. | ATR-CCR-0037 |
| Description | Fully defined, xeno-free, feeder-free medium for culture of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs). Formulated with only 8 essential components (insulin, transferrin, selenium, FGF2, TGFβ1, L-ascorbic acid, DMEM/F12 base, NaHCO₃), eliminating serum, albumin, and other undefined factors. Supports robust proliferation of pluripotent stem cells while maintaining karyotype stability and pluripotency marker expression over extended culture periods. |
| Intended Use | Feeder-free culture of human ES and iPS cells; maintenance of pluripotency; stem cell expansion for differentiation protocols; disease modeling; drug screening with pluripotent stem cells; cell therapy manufacturing research. |
| Principle / Technology | Eight chemically defined components provide essential signaling for pluripotency maintenance: FGF2 activates MAPK/ERK, TGFβ1 maintains SMAD2/3 signaling; insulin and transferrin support metabolism; ascorbic acid promotes extracellular matrix production; defined formulation eliminates lot-to-lot variability of serum and albumin-containing media |
| Detection Method | Change medium daily (2 mL per well of 6-well plate); passage cells at 70-85% confluence using EDTA or gentle dissociation reagent; split ratio 1:6 to 1:12 every 4-5 days; coat culture vessels with vitronectin or laminin-521 (not included) |
| Sample Type | Human ES cells (H1, H9, etc.) and iPS cells; not suitable for mouse ES/iPS cells which require LIF instead of FGF2/TGFβ |
| Reaction Conditions / Protocol | Culture conditions: 37 °C, 5% CO₂, 95% humidity; medium pre-warmed to RT before use; daily medium change critical — pluripotent stem cells rapidly acidify medium |
| Components / Formulation | DMEM/F12 base medium with insulin (19.4 mg/L), transferrin (10.7 mg/L), selenium (14 µg/L as sodium selenite), recombinant human FGF2 (100 µg/L), recombinant human TGFβ1 (2 µg/L), L-ascorbic acid (64 mg/L), NaHCO₃ (543 mg/L) |
| Storage Conditions | -20 °C for long-term (>1 month); 2-8 °C for up to 2 weeks; protect from light; growth factors are labile — avoid repeated freeze-thaw |
| Shelf Life | 12 months at -20 °C; use within 2 weeks after thawing at 4 °C |
| Package Specifications | 500 mL bottle; also available in 100 mL and 1,000 mL formats |
| Product Form | Sterile liquid medium, ready-to-use after thawing and supplementation (if required by specific protocol); 0.22 µm filtered |
| Key Features | Fully defined 8-component formulation; xeno-free and feeder-free; eliminates serum-related variability; supports stable karyotype over >50 passages; compatible with vitronectin and laminin-521 matrices; widely cited in stem cell literature; clinical translation-ready formulation |
| Purity | All components recombinant or synthetic; no human or animal-derived components; endotoxin <0.05 EU/mL; sterility tested per USP <71>; osmolarity 315-345 mOsm/kg |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Cold pack -20 °C; ship on dry ice for long-distance transport; do not allow to thaw during transit if -20 °C storage is required |
| Expiration Date / Stability | 12 months at -20 °C; 2 weeks at 4 °C after thawing; growth factors (FGF2, TGFβ1) are the limiting stability factors; discard if pH indicator (phenol red) shifts to yellow (acidic) or purple (alkaline) |
| Regulatory / Compliance | For laboratory and research use only; RUO; xeno-free formulation; manufactured under ISO 9001 and ISO 13485; growth factors are research-grade recombinant proteins |
| Compatibility | Compatible with vitronectin (VTN-N) and laminin-521 (LN-521) coating matrices; not compatible with Matrigel without adaptation; EDTA passaging recommended (0.5 mM EDTA in PBS, 5-8 min at RT) — avoid trypsin which dissociates pluripotent colonies into single cells causing poor survival; ROCK inhibitor (Y-27632, 10 µM) recommended for 24 hr after passaging |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
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