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| Product Name | KnockOut Serum Replacement, for ES and iPS Cells |
| Catalog No. | ATR-CCR-0024 |
| Description | A defined serum-free supplement designed to replace fetal bovine serum in the culture of mouse and human embryonic stem cells and induced pluripotent stem cells while maintaining pluripotency and normal karyotype. |
| Intended Use | Replacing FBS in ES and iPS cell culture medium to maintain undifferentiated growth, genomic stability, and pluripotency without the differentiation-inducing effects and lot-to-lot variability of serum. |
| Principle / Technology | Contains amino acids, vitamins, transferrin, insulin, selenium, and lipid-rich BSA in a formulation that supports self-renewal signaling through LIF/STAT3 (mouse) and FGF2/TGF-beta (human) pathways without serum-induced differentiation signals. |
| Detection Method | Alkaline phosphatase staining for pluripotency, Oct4/Nanog/Sox2 expression by immunostaining, karyotype analysis |
| Sample Type | Mouse embryonic stem cells, human embryonic stem cells, induced pluripotent stem cells |
| Performance Range / Specifications | Maintains >95% undifferentiated colony morphology for >10 passages; normal karyotype in >90% of metaphase spreads analyzed |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement KnockOut DMEM or DMEM/F12 with 15–20% KnockOut Serum Replacement, L-glutamine, NEAA, and 2-mercaptoethanol; add 1000 U/mL LIF for mouse ES cells or 4–10 ng/mL bFGF for human ES cells. |
| Components / Formulation | Amino acids, vitamins, transferrin or iron carrier, insulin, selenium, lipid-rich BSA (AlbuMAX), trace elements in DMEM/F12 base |
| Storage Conditions | -20°C (long term); 2–8°C after thawing, protect from light |
| Shelf Life | 24 months at -20°C; 2 weeks at 2–8°C after thawing |
| Package Specifications | 50 mL, 500 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Pluripotency maintenance validation, alkaline phosphatase activity, embryoid body formation, sterility, endotoxin |
| Key Features | Defined serum replacement; maintains pluripotency; reduces differentiation; validated for mouse and human pluripotent stem cells; consistent lot performance |
For research use only, not for clinical use.
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