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| Product Name | Collagen Type I Coating Solution (Rat Tail) |
| Catalog No. | ATR-CCR-0028 |
| Description | A purified rat tail collagen type I solution for coating tissue culture surfaces to promote cell attachment, spreading, and proliferation of primary cells and cell lines requiring extracellular matrix substrates. |
| Intended Use | Creation of biomimetic extracellular matrix coatings on tissue culture plastic and glass surfaces to enhance attachment and growth of primary cells, stem cells, and matrix-dependent cell lines. |
| Principle / Technology | Collagen type I self-assembles into a fibrillar network at neutral pH and 37°C, presenting integrin-binding motifs (RGD sequences) that mediate cell attachment through integrin receptors α1β1 and α2β1. |
| Detection Method | Cell attachment, spreading, and morphology assessed by phase-contrast microscopy; cell proliferation quantified by metabolic activity assay or cell counting |
| Sample Type | Tissue culture plasticware (flasks, multiwell plates, chamber slides) or glass coverslips for primary cell culture, stem cell culture, and cell-based assays |
| Performance Range / Specifications | Recommended coating concentration: 5–10 μg/cm² for standard coating; reconstituted solution at 3–4 mg/mL in 0.02 M acetic acid; gelation occurs at neutral pH and 37°C |
| Sensitivity / LOD | Improves attachment of matrix-dependent cell types (primary hepatocytes, endothelial cells, some epithelial lines) that otherwise fail to adhere to tissue culture plastic |
| Specificity | Type I collagen is the most abundant fibrillar collagen in connective tissues; provides specific attachment signals for cells expressing collagen-binding integrins; does not support attachment of suspension-adapted lymphocytes |
| Reaction Conditions / Protocol | Dilute collagen stock to desired concentration in sterile 0.02 M acetic acid or PBS, add sufficient volume to cover the culture surface (typical 0.5 mL per well of a 6-well plate), incubate at room temperature for 1 hour or 37°C for 30 minutes, aspirate excess solution, rinse with PBS or culture medium, seed cells |
| Components / Formulation | Rat tail collagen type I solution at 3–4 mg/mL in 0.02 M acetic acid, sterile-filtered; each lot tested for gelation and cell attachment activity |
| Storage Conditions | 2–8°C; do not freeze as freezing may cause collagen precipitation; protect from repeated temperature fluctuations |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 10 mL at 3–4 mg/mL (sufficient for coating approximately 50–100 T75 flasks at 5 μg/cm²) |
| Product Form | Sterile, viscous liquid in 0.02 M acetic acid |
| Quality Control | Each lot tested for concentration by hydroxyproline assay, sterility, endotoxin, gelation capacity at neutral pH, and promotion of cell attachment using a standard fibroblast adhesion assay |
| Key Features | Rat tail collagen provides native animal-derived ECM for authentic cell response; simple coating protocol requires no special equipment; supports a broad range of primary and established cell types; acidic storage maintains collagen solubility until neutralization |
For research use only, not for clinical use.
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