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| Product Name | Fetal Bovine Serum, Heat-Inactivated, USDA-Origin |
| Catalog No. | ATR-CCR-0009 |
| Description | Premium heat-inactivated fetal bovine serum sourced from USDA-approved abattoirs, triple 0.1 µm filtered, with complement system inactivated by controlled heating for consistent cell culture performance. |
| Intended Use | Serum supplement for mammalian cell culture media, providing essential growth factors, hormones, attachment factors, and carrier proteins required for cell proliferation and maintenance in vitro. |
| Principle / Technology | Heat treatment at 56°C for 30 minutes inactivates the complement system, reducing the risk of complement-mediated cell lysis in immunological assays; gamma irradiation further ensures viral safety. |
| Detection Method | Cell growth promotion assay with reference cell lines, total protein determination, hemoglobin spectrophotometry |
| Sample Type | All types of mammalian cell cultures: cell lines, primary cells, stem cells, hybridomas |
| Performance Range / Specifications | Total protein 3.0–4.5 g/dL; hemoglobin <20 mg/dL; endotoxin <10 EU/mL; supports cell growth equivalent to or better than reference serum |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Thaw overnight at 2–8°C; heat inactivate by incubating at 56°C for 30 min with gentle swirling every 10 min; supplement culture medium at 5–20% v/v. |
| Components / Formulation | Fetal bovine serum, heat-inactivated, triple 0.1 µm filtered, gamma irradiated, mycoplasma tested |
| Storage Conditions | -20°C (long term); 2–8°C after thawing (use within 4 weeks) |
| Shelf Life | 5 years at -20°C |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Frozen liquid |
| Quality Control | Cell growth promotion (batch release), endotoxin, hemoglobin, pH, osmolality, mycoplasma negative, viral testing per 9CFR 113.53, gamma irradiated |
| Key Features | USDA origin; heat inactivated; triple filtered; extensive QC testing; consistent lot-to-lot performance; mycoplasma and virus tested |
For research use only, not for clinical use.
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