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| Product Name | Trypsin-EDTA, 0.25%, Phenol Red, Porcine Origin |
| Catalog No. | ATR-CCR-0015 |
| Description | A porcine-derived trypsin-EDTA solution at 0.25% trypsin with 0.02% EDTA for the enzymatic detachment of adherent mammalian cells from culture vessel surfaces during routine subculturing. |
| Intended Use | Dissociation of adherent mammalian cell monolayers for passaging, cell counting, and preparation of single-cell suspensions for downstream applications. |
| Principle / Technology | Trypsin cleaves peptide bonds at the carboxyl side of lysine and arginine residues, digesting extracellular matrix and cell adhesion proteins; EDTA chelates calcium and magnesium ions, disrupting integrin-mediated cell-substrate adhesion at focal adhesions. |
| Detection Method | Cell detachment time monitoring, viability by trypan blue exclusion post-trypsinization, reattachment efficiency |
| Sample Type | Adherent mammalian cell lines: HeLa, HEK293, MRC-5, Vero, CHO, NIH/3T3, and most other fibroblast and epithelial lines |
| Performance Range / Specifications | Complete monolayer detachment within 2–5 minutes at 37°C for most cell lines; post-trypsinization viability >95% |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Wash monolayer with PBS without Ca2+ and Mg2+; add trypsin-EDTA to cover cell surface (1–2 mL per T-75 flask); incubate at 37°C until cells round up (2–5 min); neutralize with complete medium containing FBS; centrifuge and resuspend. |
| Components / Formulation | Trypsin 0.25% (1:250), EDTA 0.02% in Hank's Balanced Salt Solution without calcium, magnesium, and sodium bicarbonate, phenol red indicator |
| Storage Conditions | -20°C (long term); 2–8°C after thawing |
| Shelf Life | 24 months at -20°C; 2 months at 2–8°C |
| Package Specifications | 100 mL, 500 mL, 6 × 100 mL |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Trypsin activity by BAEE unit assay, sterility, endotoxin, cell detachment and viability testing |
| Key Features | Porcine derived; 0.25% concentration for routine use; phenol red for visualization; sterile; validated for broad cell line compatibility |
For research use only, not for clinical use.
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