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| Product Name | ITS Liquid Media Supplement (Insulin-Transferrin-Selenium) |
| Catalog No. | ATR-CCR-0030 |
| Description | A defined, serum-free cell culture supplement containing recombinant human insulin, human transferrin, and sodium selenite. The supplement supports the growth of many cell types in reduced-serum or serum-free media formulations. |
| Intended Use | Serum reduction or elimination in mammalian cell culture by providing essential growth factors and nutrients that are normally supplied by fetal bovine serum. |
| Principle / Technology | Insulin stimulates glucose and amino acid uptake, protein synthesis, and cell proliferation. Transferrin provides bioavailable iron for cellular metabolism. Selenium is an essential cofactor for glutathione peroxidase and other selenoproteins involved in antioxidant defense. |
| Detection Method | Cell proliferation measured by cell counting, metabolic activity assay (MTT, CCK-8), or protein content determination |
| Sample Type | Mammalian cell lines adapted to low-serum or serum-free culture; particularly useful for CHO, hybridoma, HEK293, and other bioproduction cell lines |
| Performance Range / Specifications | Recommended working concentrations: insulin 10 μg/mL, transferrin 5.5 μg/mL, selenium 6.7 ng/mL in final culture medium; supplied as 1,000× concentrate |
| Sensitivity / LOD | Supports cell proliferation equivalent to 5–10% serum supplementation for many cell lines when combined with appropriate basal medium and additional supplements |
| Specificity | Provides essential nutrients required by all mammalian cells; does not provide attachment factors, carrier proteins (other than transferrin), or other serum components; additional supplements may be needed for some cell types |
| Reaction Conditions / Protocol | Add 1 μL of ITS supplement per mL of culture medium (1:1,000 dilution); mix thoroughly; the supplemented medium can be used immediately or stored at 2–8°C |
| Components / Formulation | Recombinant human insulin, human holo-transferrin (iron-saturated), sodium selenite in a sterile, ready-to-use solution; endotoxin tested |
| Storage Conditions | 2–8°C protected from light; do not freeze |
| Shelf Life | 18 months from date of manufacture |
| Package Specifications | 10 mL (1,000× concentrate, sufficient for supplementing 10 L of medium) |
| Product Form | Ready-to-use sterile liquid |
| Quality Control | Each lot tested for sterility, endotoxin, and cell growth-promoting activity using a standardized CHO or hybridoma cell proliferation assay in serum-reduced medium; component concentrations verified by biochemical analysis |
| Key Features | Defined composition eliminates the variability associated with serum supplementation; 1,000× concentrate format minimizes pipetting volumes and waste; recombinant insulin eliminates animal-derived components for regulatory compliance |
For research use only, not for clinical use.
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