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| Product Name | Neurobasal Medium, Serum-Free, for Primary Neuronal Culture |
| Catalog No. | ATR-CCR-0038 |
| Description | Serum-free basal medium optimized for long-term survival and maturation of primary neuronal cell cultures from embryonic, postnatal, and adult brain tissue. Formulated with optimized amino acid concentrations, reduced osmolarity, and selenium/iron supplements to promote neuronal health while suppressing glial cell proliferation. When supplemented with B-27 (2% v/v) and GlutaMAX (0.5 mM), supports neuronal cultures for 4+ weeks with maintained electrical activity. |
| Intended Use | Primary neuronal cell culture (hippocampal, cortical, striatal, dopaminergic); long-term neuronal survival and maturation studies; neurotoxicity and neuroprotection screening; synaptic physiology and electrophysiology; neurodegenerative disease modeling. |
| Principle / Technology | Serum-free formulation selectively supports post-mitotic neurons while inhibiting glial proliferation; optimized amino acid profile (elevated cysteine, reduced glutamine) supports neuronal glutathione synthesis and reduces excitotoxicity; reduced osmolarity (235 mOsm/kg) matches cerebrospinal fluid; selenium and iron support antioxidant enzyme function |
| Detection Method | Supplement Neurobasal Medium with B-27 Supplement (2% v/v) and GlutaMAX (0.5 mM) or L-glutamine (0.5 mM) before use; add antibiotics (penicillin-streptomycin) as required; culture neurons on poly-D-lysine or poly-L-lysine coated surfaces; half-feed (replace 50% medium) every 3-4 days |
| Sample Type | Primary neurons from embryonic (E15-E18), postnatal (P0-P7), and adult rodent brain; also used for human iPSC-derived neurons; not suitable for mitotic cells or neuron-glia mixed cultures where glial support is desired (use Neurobasal-A or DMEM + serum for mixed cultures) |
| Reaction Conditions / Protocol | Culture at 37 °C, 5% CO₂; pre-equilibrate medium in incubator for 30 min before use; gentle handling essential — neurons are sensitive to temperature and pH changes |
| Components / Formulation | Neurobasal Medium base (DMEM-derived with reduced osmolarity), amino acids (optimized profile), vitamins, inorganic salts, selenium (as sodium selenite), iron (as ferric nitrate), phenol red pH indicator; B-27 supplement and GlutaMAX sold separately |
| Storage Conditions | 2-8 °C; protect from light; do not freeze; complete medium (with B-27 and GlutaMAX) stable for 2 weeks at 4 °C |
| Shelf Life | 12 months from manufacture date at 2-8 °C; use within 4 weeks after opening |
| Package Specifications | 500 mL bottle |
| Product Form | Sterile liquid medium, ready-to-use after supplementation; 0.22 µm filtered |
| Key Features | Serum-free formulation inhibits glial overgrowth; optimized for neuronal survival >4 weeks; reduced osmolarity (235 mOsm/kg) matches CSF; elevated cysteine supports glutathione synthesis; widely used in neuroscience research — >15,000 PubMed citations; compatible with B-27 and N-2 supplements; phenol red for visual pH monitoring |
| Purity | Endotoxin <0.1 EU/mL; sterility tested; osmolarity 230-245 mOsm/kg (without supplements); pH 7.4 ± 0.1 at 5% CO₂ equilibration |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze; protect from light during transit |
| Expiration Date / Stability | 12 months at 2-8 °C protected from light; after opening, use within 4 weeks; complete supplemented medium: 2 weeks at 4 °C; discard if pH indicator shifts to yellow (bacterial contamination) or medium becomes turbid |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 manufactured; animal component-free basal medium |
| Compatibility | Compatible with B-27 Supplement (2%), N-2 Supplement (1%), GlutaMAX (0.5 mM), and penicillin-streptomycin; for optimal neuronal survival, use freshly supplemented medium; half-feed rather than full medium change minimizes disruption of neuronal conditioning factors; for embryonic neurons, use at plating and replace 50% at day 1 and every 3-4 days thereafter |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
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