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| Product Name | GlutaMAX Supplement (L-Alanyl-L-Glutamine Dipeptide) |
| Catalog No. | ATR-CCR-0029 |
| Description | A stable dipeptide form of L-glutamine (L-alanyl-L-glutamine) for supplementation of cell culture media. Unlike free L-glutamine, the dipeptide is resistant to thermal degradation and does not spontaneously break down to form ammonia, providing more consistent glutamine availability. |
| Intended Use | Glutamine supplementation for mammalian cell culture media, replacing free L-glutamine to improve media stability and reduce toxic ammonia accumulation during extended culture. |
| Principle / Technology | The L-alanyl-L-glutamine dipeptide is stable in aqueous solution at 37°C; cells utilize membrane-bound peptidases to cleave the dipeptide, releasing L-glutamine and L-alanine at a controlled rate that matches cellular metabolic demand. |
| Detection Method | Cell proliferation, viability, and metabolism assessed by cell counting, metabolic activity assays, and spent media analysis for ammonia and glutamine concentrations |
| Sample Type | All glutamine-requiring mammalian cell lines (most adherent and suspension cell lines); especially beneficial for ammonia-sensitive cell types and long-term cultures |
| Performance Range / Specifications | Typical supplementation: 2–6 mM final concentration in complete medium (equivalent to 2–6 mM L-glutamine); supplied as 200 mM concentrate (100×) in 0.85% NaCl |
| Sensitivity / LOD | Supports cell growth at the same concentration as free L-glutamine with equivalent or improved cell yields |
| Specificity | Provides L-glutamine to all glutamine-requiring mammalian cells; the dipeptide cleavage mechanism ensures glutamine delivery without the spontaneous degradation that limits free glutamine media shelf life |
| Reaction Conditions / Protocol | Add 5–10 mL of 200 mM GlutaMAX per 500 mL of glutamine-free basal medium to achieve a final concentration of 2–4 mM; mix thoroughly; supplemented medium can be stored at 2–8°C for up to 6 weeks without significant glutamine loss |
| Components / Formulation | 200 mM L-alanyl-L-glutamine in 0.85% NaCl, sterile-filtered and cell culture tested |
| Storage Conditions | 2–8°C protected from light; stable at this temperature for the expiration period indicated on the label |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 100 mL (200 mM concentrate, sufficient for supplementing 10–20 L of complete medium) |
| Product Form | Ready-to-use sterile liquid in PETG bottle |
| Quality Control | Each lot tested for sterility, endotoxin, pH, osmolality, and cell growth-promoting activity using a standardized glutamine-dependent cell line proliferation assay; dipeptide purity >99% by HPLC |
| Key Features | Thermal and chemical stability eliminates the glutamine degradation issues that limit the shelf life of glutamine-supplemented media; reduced ammonia accumulation enhances cell viability in long-term cultures; the controlled enzymatic cleavage mimics physiological glutamine availability |
For research use only, not for clinical use.
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