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| Product Name | DMEM/F12 Medium with HEPES, No Phenol Red |
| Catalog No. | ATR-CCR-0033 |
| Description | A 1:1 mixture of DMEM and Ham's F12 nutrient mixture supplemented with 15 mM HEPES buffer and prepared without phenol red pH indicator. The HEPES buffer provides additional pH stability outside the CO₂ incubator during cell manipulation and microscopy procedures. The absence of phenol red eliminates estrogenic activity and reduces background fluorescence, making this formulation ideal for hormone-responsive cell studies and fluorescence microscopy applications. |
| Intended Use | Culture of cells requiring enriched nutrient formulation with CO₂-independent pH buffering for fluorescence imaging workflows. |
| Principle / Technology | DMEM/F12 hybrid medium with HEPES organic buffer for atmospheric CO₂ pH stability |
| Detection Method | Fluorescence microscopy with reduced background; hormone response assays without phenol red interference |
| Sample Type | MCF-7 breast cancer cells, steroid hormone-responsive cells, cells used for fluorescence microscopy |
| Performance Range / Specifications | HEPES: 15 mM; phenol red: absent; L-glutamine: 2.5 mM; sodium pyruvate: 0.5 mM |
| Sensitivity / LOD | pH drift ≤0.2 units over 4 hr in ambient atmosphere at room temperature |
| Specificity | DMEM/F12 nutrient profile supports a broader range of cell types than either parent medium alone |
| Reaction Conditions / Protocol | Supplement with 5–10% charcoal-stripped FBS for hormone studies and 1% antibiotics; incubate at 37 °C in 5% CO₂; HEPES buffering provides short-term pH stability outside incubator |
| Components / Formulation | DMEM/F12 with 15 mM HEPES, without phenol red, sterile-filtered |
| Storage Conditions | 2–8 °C, protected from light |
| Shelf Life | 12 months at 2–8 °C |
| Package Specifications | 500 mL |
| Product Form | Liquid, sterile, ready-to-use |
| Quality Control | Sterility and endotoxin tested per standard specifications; pH verified at 7.4; osmolality within 290–320 mOsm/kg |
| Key Features | Phenol red-free formulation critical for estrogen receptor studies and reduces autofluorescence in live-cell imaging |
| Purity | Sterile; endotoxin ≤0.25 EU/mL; mycoplasma-free |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Ambient temperature for dry powder; cold packs for liquid media |
| Expiration Date / Stability | 12 months for liquid media; 24–36 months for dry powder |
| Regulatory / Compliance | Research use; GMP-grade options available; Certificate of Origin for animal-derived components |
| Compatibility | Compatible with standard mammalian cell culture incubator conditions (37 °C, 5% CO₂) |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
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