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| Product Name | MEM Non-Essential Amino Acids, 100x Solution |
| Catalog No. | ATR-CCR-0011 |
| Description | A 100-fold concentrated sterile solution containing seven non-essential amino acids for supplementation of MEM and other basal media to improve cell growth, viability, and protein synthesis. |
| Intended Use | Supplementing basal cell culture media with non-essential amino acids to reduce the metabolic burden on cells to synthesize these compounds, improving growth rates and reducing cell stress. |
| Principle / Technology | Provision of L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycine, L-proline, and L-serine in pre-formed form eliminates the energy and nitrogen costs of their biosynthesis, improving cell growth efficiency. |
| Detection Method | Cell growth rate and viability comparison with unsupplemented controls, protein synthesis rate assessment |
| Sample Type | Cell lines cultured in MEM, DMEM, or RPMI base media, particularly fastidious lines and primary cells |
| Performance Range / Specifications | Each amino acid at 0.1 mM final concentration at 1:100 dilution; growth rate increase 10–30% for auxotrophic and fastidious cells |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution); stable throughout medium shelf life at 2–8°C. |
| Components / Formulation | L-Alanine, L-Asparagine, L-Aspartic Acid, L-Glutamic Acid, Glycine, L-Proline, L-Serine at 10 mM each in MEM, sterile-filtered |
| Storage Conditions | 2–8°C |
| Shelf Life | 18 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Amino acid concentration verification by HPLC, sterility, endotoxin, cell growth enhancement validation |
| Key Features | Sterile 100x concentrate; improves growth of fastidious cells; reduces biosynthetic burden; ready to use; standard supplement for MEM-based media |
For research use only, not for clinical use.
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