Dimethyl Sulfoxide (DMSO), Anhydrous, Hybri-Max Grade, ≥99.9%

Name: Dimethyl Sulfoxide (DMSO), Anhydrous, Hybri-Max Grade, ≥99.9%
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Cat.No: ATR-SDS-0025 Datasheet

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Product Name	Dimethyl Sulfoxide (DMSO), Anhydrous, Hybri-Max Grade, ≥99.9%
Catalog No.	ATR-SDS-0025
Description	High-purity anhydrous dimethyl sulfoxide (DMSO) for solubilization of hydrophobic compounds, cryopreservation of cells, and as a vehicle solvent for drug discovery and cell-based assays. Hybri-Max grade ensures low endotoxin (<0.1 EU/mL), low water content (<0.005%), and absence of cytotoxic impurities that could affect hybridoma, stem cell, or primary cell culture performance. Triple-distilled under inert atmosphere and packaged under argon in amber glass ampoules or septum-sealed bottles to maintain anhydrous condition throughout storage.
Intended Use	Solubilization of water-insoluble small molecule compounds, peptides, and fluorescent probes for cell-based assays; cryoprotectant for mammalian cell freezing; vehicle control solvent in drug screening at final concentration ≤0.1% v/v.
Principle / Technology	Dipolar aprotic solvent dissolves polar and nonpolar compounds; high dielectric constant (ε = 46.7) enables solvation of charged species; cryoprotective effect by reducing ice crystal formation during controlled-rate freezing.
Detection Method	Dissolve compound in anhydrous DMSO to desired stock concentration (typically 1-100 mM); dilute into aqueous assay buffer or culture medium at ≤0.1% final DMSO concentration; include vehicle-only control.
Sample Type	Hydrophobic small molecules, peptides, fluorescent dyes, lipophilic probes, cryopreserved cells.
Performance Range / Specifications	Solubility enhancement: 10-1000 fold for hydrophobic compounds compared to aqueous buffer; DMSO stock solutions stable at -20 °C for months to years for most compounds.
Sensitivity / LOD	Water content <0.005% by Karl Fischer titration; endotoxin <0.1 EU/mL; residual solvents below ICH Q3C limits.
Specificity	DMSO is a universal solvent for diverse chemical classes; potential for nonspecific effects at concentrations >0.5% v/v; not recommended for functional assays at >0.1% v/v due to possible solvent artifacts.
Reaction Conditions / Protocol	DMSO is chemically inert under recommended storage and use conditions; avoid strong oxidizers, acid chlorides, and reducing agents.
Components / Formulation	Dimethyl sulfoxide, ≥99.9% purity, anhydrous.
Storage Conditions	Store at room temperature (15-25 °C) in original container under inert atmosphere; protect from moisture; DMSO is hygroscopic.
Shelf Life	36 months from date of manufacture in unopened sealed ampoule.
Package Specifications	10 × 1 mL amber glass ampoules; 50 mL, 100 mL, 500 mL septum-sealed amber bottles.
Product Form	Clear, colorless, hygroscopic liquid with characteristic slight sulfurous odor.
Quality Control	Each lot tested for purity by GC (>99.9%), water content (Karl Fischer), endotoxin (LAL), UV absorbance at 260/280 nm, and cytotoxicity in CHO-K1 cells (IC50 >2% v/v).
Key Features	Anhydrous and Hybri-Max grade; triple-distilled; argon-packed; low endotoxin; low water content; cell culture tested; amber glass packaging for light protection; septum-sealed for syringe access.
Purity	≥99.9% by GC; individual impurity <0.05%.
Concentration	Neat (100%) liquid; typical working concentration ≤0.1% v/v in cell culture.
Activity / Unit Definition	Not applicable — inert solvent without enzymatic or biological activity per se.
Molecular Weight	78.13 g/mol ((CH3)2SO).
Source / Origin	Synthetic from dimethyl sulfide oxidation; triple-distilled under reduced pressure.
pH Range / Optimal pH	Neutral; aqueous solutions pH 6.5-7.5.
Shipping Conditions	Ambient temperature; protect from moisture.
Expiration Date / Stability	36 months at room temperature in unopened ampoule; after opening, use within 6 months and store under dry inert gas.
Regulatory / Compliance	For research use only; not for therapeutic or diagnostic applications. Hybri-Max grade certified for hybridoma and cell culture use.
Compatibility	Compatible with most organic and aqueous solvents. Incompatible with strong oxidizers (risk of explosion), acetyl halides, and active metals. DMSO penetrates skin and nitrile gloves readily — use appropriate PPE.
Recommended Buffer System	Not applicable — use as neat solvent.
Application Notes / Precautions	Pre-warm DMSO to room temperature before opening to prevent moisture condensation. For anhydrous work, use oven-dried glass syringes and needles to transfer DMSO. Aliquot into single-use vials to minimize moisture exposure. DMSO freezes at 18.5 °C — if frozen, thaw at room temperature. For cell-based assays, keep final DMSO concentration ≤0.1% v/v; include DMSO vehicle control at same concentration. Store compound-DMSO stocks at -20 °C in sealed vials with desiccant.
Batch-to-Batch Consistency	Purity ≥99.9% by GC; water content <0.005%; endotoxin <0.1 EU/mL; UV cutoff <268 nm at absorbance 1.0.

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For research use only, not for clinical use.