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| Product Name | Warm-Start Reverse Transcriptase |
| Catalog No. | IEC-HMM-0100 |
| Description | A warm-start reverse transcriptase engineered for rapid, high-temperature cDNA synthesis and custom one-step RT-qPCR formulation. |
| Intended Use | Custom one-step RT-qPCR systems; rapid reverse transcription; high-temperature cDNA synthesis. |
| Principle / Technology | Warm-start behavior limits nonspecific reverse transcription during low-temperature setup and instrument ramping, while enhanced thermostability supports efficient cDNA synthesis at elevated temperatures. |
| Detection Method | Used in one-step RT-qPCR systems with dye- or probe-based fluorescence detection. |
| Sample Type | RNA targets used in custom one-step RT-qPCR workflows. |
| Performance Range / Specifications | 10,000 U or 40,000 U; reverse transcriptase concentration 200 U/µL; rapid reverse transcription at 55°C for 2 minutes showed performance comparable to a 55°C, 15-minute program in the reported evaluation. |
| Sensitivity / LOD | Supports efficient cDNA synthesis from both low- and high-copy RNA templates. |
| Specificity | Warm-start control reduces nonspecific low-temperature reverse transcription and preserves reaction substrates for target amplification. |
| Reaction Conditions / Protocol | Use with the supplied 5× reverse-transcription buffer in a custom one-step RT-qPCR formulation. A rapid 55°C, 2-minute reverse-transcription step can be evaluated for compatible assays. |
| Components / Formulation | 5× warm-start reverse-transcription buffer and reverse transcriptase at 200 U/µL. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 10,000 U; 40,000 U. |
| Product Form | Reverse transcriptase with separate 5× reaction buffer. |
| Quality Control | High- and low-template balance, rapid reverse-transcription performance and accelerated stability are evaluated. |
| Key Features | Warm-start activity; rapid 2-minute reverse transcription; high-temperature compatibility; balanced low- and high-copy performance; strong storage stability. |
| Concentration | 200 U/µL. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported performance remained stable after 50 liquid-nitrogen freeze–thaw cycles, 4 weeks at 4°C and 30 days at 37°C. |
| Compatibility | Designed for custom one-step RT-qPCR formulations and rapid cycling workflows. |
For research use only, not for clinical use.
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