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| Product Name | High Affinity Porcine RNase Inhibitor, Ultra-Low Residual (40 U/uL) |
| Catalog No. | IVDRM-0583 |
| Description | A recombinant porcine RNase inhibitor expressed and purified from E. coli, this 40 U/uL preparation binds RNase A, RNase B and RNase C non-covalently to form an inactive complex and is compatible with reverse transcriptases and DNA polymerases. It is effective against both RNA and total RNA inputs, and 8 U per reaction effectively inhibits 50 ng of RNase A. The ultra-low residual process keeps host genomic DNA below 0.1 copies per 100 U with no exonuclease, nickase or RNase residue, which suits RNA-seq library construction and single cell or low input RNA workflows where background must stay minimal. |
| Intended Use | RNA protection in RT-qPCR, RT-PCR, NGS library construction and low input or single cell RNA workflows |
| Principle / Technology | Non-covalent complex formation with RNase A, B and C that inactivates the enzymes |
| Sample Type | RNA and total RNA inputs in reverse transcription and library preparation |
| Specificity | Host genomic DNA below 0.1 copies per 100 U; 8 U inhibits 50 ng of RNase A |
| Reaction Conditions / Protocol | Add to the reaction system at the working concentration to protect RNA templates |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2 KU; 5.5 KU; 20 KU; 100 KU |
| Key Features | Ultra-low host residue; suited to NGS and low input RNA applications |
| Concentration | 40 U/uL |
| Recommended Buffer System | Hepes-KOH, KCl, DTT and Triton X-100 with 50% glycerol at pH 7.5 |
| Readouts / Downstream Assays | RT-qPCR and library construction performance with protected RNA |
For in vitro diagnostic use.
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