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| Product Name | ≥500,000 U/mL PNGase F for Broad N-Glycan Release from Glycoproteins |
| Catalog No. | IEC-HMM-0238 |
| Description | Alta DiagnoTech peptide N-glycosidase F is a high-purity liquid amidase for releasing high-mannose, hybrid and complex N-linked glycans from glycoproteins. |
| Intended Use | For removal of N-linked glycans from glycoproteins. |
| Performance Range / Specifications | No exoglycosidase, endoglycosidase F1, F2, F3, endoglycosidase H or protease activity is present. |
| Storage Conditions | Store without agitation at -25°C to -15°C. |
| Product Form | Liquid enzyme |
| Purity | ≥95% by SDS-PAGE |
| Concentration | Specific activity: ≥500,000 U/mL |
| Activity / Unit Definition | One unit is the amount of enzyme required to remove more than 95% of carbohydrate from 10 µg of denatured RNase B in a 10 µL reaction at 37°C for 1 hour. |
| Molecular Weight | 35 kDa by SDS-PAGE |
| Source / Origin | Microbial |
| pH Range / Optimal pH | Optimum pH: 7.0-8.0 |
| Application Notes / Precautions | Cleaves N-linked glycans except those carrying an alpha1-3-linked fucose on the core GlcNAc. A 20 µL reaction containing 1 µL of enzyme can be inactivated at 75°C for 10 minutes. |
| Appearance | Colorless liquid |
| Other Name | Peptide N-glycosidase F |
| Product Type | N-glycan-releasing amidase |
| Function / Efficacy | Cleaves the glycosidic linkage between the innermost N-acetylglucosamine and asparagine while keeping the released N-glycan intact. |
| Abbreviation | PNGase F |
| EC Number | EC 3.5.1.52 |
| Specific Activity | ≥500,000 U/mL |
| Isoelectric Point | 8.14 |
| Inhibitors | SDS |
| Optimal Temperature | 65°C |
| Activator | DTT |
For research use only, not for clinical use.
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