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| Product Name | >=10 U/mg Alpha-Glucosidase for 405 nm PNPG Amylase Assays |
| Catalog No. | IEC-HMM-0147 |
| Description | Alta DiagnoTech alpha-glucosidase is a pale-yellowish white amorphous enzyme powder for amylase biochemical reagent development. |
| Intended Use | Enzyme raw material for amylase biochemical assay reagents. |
| Principle / Technology | The enzyme releases p-nitrophenol from a chromogenic alpha-D-glucoside substrate; activity is calculated from the absorbance-change rate at 405 nm. |
| Detection Method | Kinetic p-nitrophenol absorbance assay at 405 nm |
| Performance Range / Specifications | Specific activity >=10 U/mg; assay linear range 100-800 U/L; recommended sample range 200-400 U/L. |
| Reaction Conditions / Protocol | Prepare the assay reagent by dissolving 13.6 g potassium dihydrogen phosphate in 800 mL water, adjusting to pH 7.0 at 25 degrees C with KOH, adding 1.50 g 4-nitrophenyl-alpha-D-glucopyranoside and 1 g sodium azide, adjusting to pH 7.00 +/- 0.05 and bringing to 1 L. Preincubate 0.900 mL assay reagent for 60 seconds at 37 degrees C, add 0.018 mL enzyme sample, mix, and after 1 minute determine the absorbance-change rate from 180 to 240 seconds at 405 nm. Activity in U/L equals Delta A/min x 2,818 x dilution factor. |
| Storage Conditions | Store at -20 degrees C |
| Shelf Life | At least 1 year at -20 degrees C |
| Product Form | Pale-yellowish white amorphous powder |
| Activity / Unit Definition | One unit is the amount of enzyme that produces 1 umol p-nitrophenol per minute under the stated reaction conditions. |
| Molecular Weight | Approximately 65 kDa |
| pH Range / Optimal pH | Stable at pH 5.5-9.5; optimum pH 7.5 |
| Recommended Buffer System | Sample diluent: dissolve 27.2 g potassium dihydrogen phosphate in 800 mL water, adjust toward pH 7.0 with KOH, add 0.37 g EDTA disodium salt, 3.00 g Thesit and 1.00 g sodium azide, adjust to pH 7.00 +/- 0.05 at 25 degrees C and bring to 1 L. |
| Appearance | Pale-yellowish white amorphous powder |
| Alias | Alpha-Glucosidase |
| Product Type | Alpha-glucosidase enzyme raw material |
| Function / Efficacy | Hydrolyze alpha-D-glucoside substrates to release p-nitrophenol in amylase assay systems. |
| Abbreviation | AGH |
| Customization | Specifications can be customized |
| Specific Activity | >=10 U/mg |
| Isoelectric Point | 5.2 |
| Inhibitors | Not inhibited by sodium azide |
| Optimal Temperature | >65 degrees C |
| Thermal Stability | Stable below 55 degrees C |
| Michaelis Constant | 6.3 x 10^-4 M for PNPG |
| Detection Wavelength | 405 nm |
| Linear Range | 100-800 U/L |
For research use only, not for clinical use.
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