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| Product Name | High-Sensitivity Warm-Start Bst III DNA Polymerase, Glycerol-Free |
| Catalog No. | IEC-HMM-0110 |
| Description | A glycerol-free warm-start Bst DNA polymerase engineered for high-sensitivity isothermal amplification and freeze-dried reagent development. |
| Intended Use | LAMP, RT-LAMP and related isothermal amplification assays. |
| Principle / Technology | The enzyme retains 5′→3′ polymerase activity and strong strand-displacement activity while lacking 5′→3′ exonuclease activity. An auxiliary factor increases LAMP sensitivity, and warm-start control suppresses activity below 50°C. |
| Detection Method | Endpoint, colorimetric or real-time fluorescent isothermal amplification. |
| Sample Type | Purified nucleic acids and compatible crude lysates, including saliva and nasal or throat swab lysates. |
| Performance Range / Specifications | 1,600 U or 8,000 U; reported sensitivity improvement of approximately 10- to 100-fold in LAMP applications; supports room-temperature setup. |
| Sensitivity / LOD | Designed for improved detection of low-copy templates. |
| Specificity | Warm-start inhibition below 50°C reduces nonspecific amplification during reaction setup. |
| Reaction Conditions / Protocol | Combine with isothermal amplification buffer, magnesium, dNTPs, assay primers and template. Incubate above 50°C using conditions optimized for the target assay. |
| Components / Formulation | Glycerol-free Bst III DNA polymerase with an amplification-enhancing auxiliary factor; no lyophilization bulking agent is included. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 1,600 U; 8,000 U. |
| Product Form | Glycerol-free warm-start strand-displacing DNA polymerase. |
| Quality Control | Low-template sensitivity, amplification speed, crude-lysate tolerance and warm-start control are evaluated. |
| Key Features | High sensitivity; rapid amplification; strong strand displacement; crude-sample tolerance; room-temperature setup; lyophilization compatible. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for LAMP and RT-LAMP formulations, including custom freeze-dried reagents. |
| Application Notes / Precautions | Add suitable excipients separately when developing a lyophilized product. |
For research use only, not for clinical use.
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