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| Product Name | Recombinant DNase I, E. coli Expressed, RNase-Free |
| Catalog No. | IVDRM-0432 |
| Description | This recombinant deoxyribonuclease I, expressed in Escherichia coli, hydrolyses phosphodiester bonds in single and double stranded DNA and releases oligodeoxynucleotides carrying 5 prime phosphate and 3 prime hydroxyl groups. Incubation at 37°C degrades DNA quickly and efficiently, and the enzyme acts on ssDNA, dsDNA, chromatin and DNA-RNA hybrids while leaving RNA untouched. Verified RNase-free, it supports DNA contamination removal during RNA extraction, template elimination after in vitro transcription and rRNA removal in RNA library construction. Optimal activity lies at pH 7 to 8, depends on calcium ions and can be stimulated by cobalt, manganese and zinc ions. |
| Intended Use | DNA contamination removal in RNA extraction, DNA template removal after in vitro transcription and rRNA removal in RNA NGS library construction |
| Principle / Technology | Endonucleolytic hydrolysis of DNA phosphodiester bonds with calcium dependent activity |
| Specificity | Removes DNA specifically without affecting RNA; acts on ssDNA, dsDNA, chromatin and DNA-RNA hybrids |
| Reaction Conditions / Protocol | Incubate at 37°C for rapid and efficient DNA degradation |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5 x 1000 U; 5000 U |
| Quality Control | RNase-free verified |
| Key Features | Broad substrate coverage including chromatin and DNA-RNA hybrids; multi pack format for routine workflows |
| pH Range / Optimal pH | Optimal pH range 7 to 8 |
| Readouts / Downstream Assays | Agarose gel confirming DNA removal without RNA degradation |
For in vitro diagnostic use.
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