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| Product Name | Fast Restriction Enzyme SphI (Recognition Site: GCATG/C) |
| Catalog No. | IVDRM-0081 |
| Description | A recombinant fast restriction enzyme engineered for rapid, precise DNA cleavage within 5–15 minutes. It is well suited for digesting plasmid DNA, PCR products, and genomic DNA. All enzymes in this series share a single universal reaction buffer, which greatly simplifies digestion setup, and exhibit generous activity headroom to handle excess substrate or difficult templates with ease. |
| Intended Use | Intended for rapid restriction digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and other molecular biology research applications. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering. It specifically recognizes the sequence GCATG/C and cleaves double-stranded DNA at defined positions within 5–15 minutes. All enzymes in this series share one universal digestion buffer, simplifying reaction setup, and offer ample activity redundancy for challenging templates or excess substrate. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete, precise DNA digestion within 5–15 minutes; enzyme concentration 20 U/μL; shared universal digestion buffer across the series; high activity redundancy for excess substrate or difficult templates. |
| Specificity | Highly sequence-specific cleavage: 5'-GCATG↓C-3' 3'-C↑GTACG-5' |
| Reaction Conditions / Protocol | Recommended reaction conditions: 1× universal fast digestion buffer Optimal incubation temperature: 37°C Typical digestion time: 5–15 min |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 50 T |
| Concentration | 20 U/μL |
| Activity / Unit Definition | Enzyme activity: 20 U/μL. |
| Source / Origin | Recombinant enzyme produced by genetic engineering (recombinant expression). |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | BbuI, PaeI, SpaHI |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying reaction setup and enabling convenient double or multiple digests. |
| Application Notes / Precautions | EcoBI methylation may overlap the recognition sequence and blocks cleavage; verify the methylation status of the template. For complete digestion of excess substrate or difficult templates, extend the incubation time or increase the enzyme amount appropriately. |
| Recognition Site | 5'-GCATG↓C-3' 3'-C↑GTACG-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | EcoBI methylation: the modification site may overlap the recognition sequence; cleavage is blocked. |
For research use only, not for clinical use.
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