- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Warm-Start Bst II DNA Polymerase Large Fragment |
| Catalog No. | IEC-HMM-0111 |
| Description | A warm-start Bst DNA polymerase large fragment with strong strand-displacement activity for rapid isothermal amplification. |
| Intended Use | LAMP, helicase-dependent amplification, rolling-circle amplification and other isothermal DNA amplification workflows. |
| Principle / Technology | The engineered large fragment provides 5′→3′ DNA polymerase and strand-displacement activities without 5′→3′ exonuclease activity. Activity is suppressed below 50°C and rapidly released above 50°C. |
| Detection Method | Endpoint or real-time isothermal amplification, depending on the assay readout. |
| Sample Type | Purified DNA and compatible crude lysates, including saliva, oral swabs and whole-blood lysates. |
| Performance Range / Specifications | 1,600 U or 8,000 U; reported detection of 10^3 copies per reaction of lambda DNA; stable after up to 3 hours of room-temperature setup. |
| Sensitivity / LOD | Reported detection of approximately 10^3 copies per reaction in a lambda DNA LAMP assay. |
| Specificity | Warm-start regulation suppresses low-temperature polymerase activity and reduces nonspecific amplification. |
| Reaction Conditions / Protocol | Prepare the reaction with a compatible isothermal buffer, magnesium, dNTPs, primers and template, then incubate above 50°C at the validated assay temperature. |
| Components / Formulation | Warm-start Bst II DNA polymerase large fragment and compatible isothermal amplification reagents as required by the assay. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 1,600 U; 8,000 U. |
| Product Form | Warm-start strand-displacing DNA polymerase. |
| Quality Control | Amplification speed, sensitivity, specificity, salt tolerance, thermal stability and crude-sample compatibility are evaluated. |
| Key Features | Fast isothermal amplification; strong strand displacement; warm-start control; high impurity tolerance; room-temperature setup. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for LAMP, HDA and RCA assays. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
