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| Product Name | Uracil DNA Glycosylase (UDG/UNG), Heat-Labile, Ultra-Clean (1 U/μL) |
| Catalog No. | IVDRM-0275 |
| Description | Heat-labile Uracil DNA Glycosylase (UDG/UNG) catalyzes the hydrolysis of the N-glycosidic bond between the uracil base and the sugar-phosphate backbone of uracil-containing DNA strands, releasing free uracil. Unlike conventional UDG, this heat-labile enzyme can be completely inactivated by incubation at 55 °C for 5 min or 50 °C for 10 min, eliminating the risk of residual activity degrading dU-containing amplification products after inactivation. The enzyme is manufactured with an ultra-clean, low nucleic acid background process that keeps E. coli host DNA carryover extremely low, making it well suited for demanding applications such as pathogen detection. |
| Intended Use | Prevention of PCR/RT-qPCR carryover contamination by degrading dUTP-containing amplicons from previous reactions; ideal for molecular diagnostic reagent development, especially pathogen detection requiring ultra-low nucleic acid background. |
| Principle / Technology | The enzyme excises uracil from uracil-containing DNA by hydrolyzing the N-glycosidic bond linking uracil to the deoxyribose-phosphate backbone, generating abasic sites that block amplification of carryover dU-containing DNA. Its heat-labile nature allows complete thermal inactivation before amplification, so newly synthesized dU-containing products remain intact for downstream analysis. |
| Detection Method | qPCR, RT-qPCR |
| Performance Range / Specifications | Ultra-clean, low nucleic acid background: E. coli genomic DNA residual < 0.1 copies per 1 U Low nuclease background: free of detectable exonuclease, nicking enzyme and RNase residuals Strong digestion capacity: 0.05 U per test fully digests 10^5 copies per test of dU-DNA products Excellent heat lability: completely inactivated at 50 °C for 10 min, 55 °C for 5 min, or ≥95 °C for 5 min Compatible with RT-qPCR systems: no impact on the detection system at a high input of 2 U per 20 μL reaction |
| Sensitivity / LOD | E. coli genomic DNA residual < 0.1 copies/U |
| Reaction Conditions / Protocol | Compatible with qPCR and RT-qPCR master mixes; no inhibition of the detection system observed at up to 2 U per 20 μL reaction. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 100 U; 500 U; 10,000 U |
| Product Form | Liquid |
| Quality Control | Protein purity ≥95% (SDS-PAGE) Free of detectable exonuclease, nicking enzyme and RNase residuals E. coli genomic DNA residual < 0.1 copies/U Functional digestion and heat-inactivation performance verified by qPCR |
| Purity | ≥95% (SDS-PAGE) |
| Concentration | 1 U/μL |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Regulatory / Compliance | IVD raw material for molecular diagnostic reagent manufacturing; for research and manufacturing use only. |
| Compatibility | Compatible with qPCR and RT-qPCR reaction systems, including one-step RT-qPCR mixes. |
| Application Notes / Precautions | The enzyme is temperature-sensitive; apply a validated heat-inactivation step (50 °C for 10 min, 55 °C for 5 min, or ≥95 °C for 5 min) before amplification to avoid degradation of dU-containing products. |
| Heat Inactivation | 50 °C for 10 min; 55 °C for 5 min; or ≥95 °C for 5 min (complete inactivation under any of these conditions) |
For research use only, not for clinical use.
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