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| Product Name | Uracil DNA Glycosylase (UDG/UNG) (1 U/μL) |
| Catalog No. | IVDRM-0274 |
| Description | Uracil DNA Glycosylase (UDG/UNG) (1 U/μL) is an anti-contamination enzyme that catalyzes the hydrolysis of the N-glycosidic bond between the uracil base and the sugar-phosphate backbone in uracil-containing DNA strands, releasing free uracil. The enzyme acts on both single-stranded and double-stranded DNA containing dU, with no activity on RNA. It is used to remove carryover (aerosol) contamination from dU-containing PCR products and to excise uracil bases from single- or double-stranded DNA. |
| Intended Use | Carryover contamination control in PCR/qPCR workflows using dUTP; removal of uracil bases from single- or double-stranded DNA. |
| Principle / Technology | UDG catalyzes hydrolysis of the N-glycosidic bond linking uracil to the deoxyribose-phosphate backbone of dU-containing DNA, generating abasic (AP) sites and releasing free uracil. Abasic DNA can no longer serve as an amplification template, thereby eliminating carryover contamination from previously amplified dU-containing PCR products. |
| Detection Method | PCR/qPCR carryover contamination control |
| Sample Type | Single-stranded or double-stranded DNA containing dU |
| Performance Range / Specifications | Concentration: 1 U/μL Removes aerosol/carryover contamination from dU-containing PCR products Removes uracil bases from single-stranded or double-stranded DNA No activity on RNA |
| Specificity | Acts specifically on uracil in single-stranded and double-stranded DNA; no activity on RNA. |
| Components / Formulation | Supplied as a concentrated enzyme solution; formulation details available on request. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years |
| Package Specifications | 100 U; 500 U; 10,000 U |
| Product Form | Liquid |
| Concentration | 1 U/μL |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15°C. |
| Regulatory / Compliance | For research use and IVD raw material manufacturing. |
| Compatibility | Compatible with PCR/qPCR systems using dUTP in place of dTTP for carryover contamination control. |
| Application Notes / Precautions | Use together with dUTP-containing amplification systems so that carryover products can be selectively degraded prior to amplification. |
For research use only, not for clinical use.
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