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| Product Name | Uracil DNA Glycosylase (UDG), Heat-Labile (Glycerol-Free) |
| Catalog No. | IVDRM-0251 |
| Description | This glycerol-free heat-labile Uracil DNA Glycosylase (UDG) is designed for the formulation of lyophilized (freeze-dried) reaction systems. The enzyme catalyzes the hydrolysis of the N-glycosidic bond between the uracil base and the sugar-phosphate backbone of uracil-containing DNA, releasing free uracil. Compared with conventional UDG, this heat-labile enzyme avoids the degradation of dU-containing amplification products at room temperature caused by residual activity that may persist after inactivation of ordinary UDG. The enzyme works at room temperature, is temperature-sensitive and is easily inactivated. |
| Intended Use | Removal of aerosol contamination from dU-containing PCR amplification products in dUTP-based PCR/qPCR systems Removal of uracil bases from single-stranded or double-stranded DNA Formulation of lyophilized (freeze-dried) reaction systems |
| Principle / Technology | UDG excises uracil from DNA by hydrolyzing the N-glycosidic bond linking the uracil base to the deoxyribose-phosphate backbone, generating apyrimidinic (AP) sites. When PCR is performed with dUTP instead of dTTP, contaminating carryover amplicons from previous reactions are degraded by a UDG pre-treatment step, while the natural thymine-containing template remains unaffected. |
| Performance Range / Specifications | Glycerol-free formulation, suitable for preparation of lyophilized reaction systems Heat-labile: temperature-sensitive and easily inactivated Removes uracil bases from single-stranded or double-stranded DNA |
| Specificity | Specifically excises uracil bases from single-stranded and double-stranded DNA; does not act on natural thymine-containing DNA templates. |
| Reaction Conditions / Protocol | Active at room temperature. A UDG pre-treatment step is performed before amplification in dUTP-based PCR/qPCR systems. |
| Components / Formulation | Glycerol-free formulation; suitable for the preparation of lyophilized systems. |
| Storage Conditions | Store at 4 °C. |
| Shelf Life | 1 year when stored at 4 °C. |
| Package Specifications | 100 U 500 U 5 KU 10 KU |
| Product Form | Liquid, glycerol-free (lyophilization-ready) |
| Expiration Date / Stability | Stable for 1 year at 4 °C. |
| Application Notes / Precautions | Unlike conventional UDG, this heat-labile enzyme eliminates the risk of residual activity degrading dU-containing amplification products at room temperature after the reaction. Use in dUTP-based amplification systems for carryover contamination control. The glycerol-free format is intended for lyophilized system formulation. |
| Recognition Site | Uracil bases in single-stranded or double-stranded DNA (cleaves the N-glycosidic bond between uracil and the sugar-phosphate backbone) |
| Heat Inactivation | Heat-labile; temperature-sensitive and readily inactivated by elevated temperature |
For research use only, not for clinical use.
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