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| Product Name | Thermostable Long-Range Reverse Transcriptase |
| Catalog No. | IEC-HMM-0101 |
| Description | A thermostable, high-processivity reverse transcriptase for efficient first-strand cDNA synthesis from structured, inhibitor-containing and long RNA templates. |
| Intended Use | Reverse transcription; first-strand cDNA synthesis; RNA-virus detection; two-step RT-qPCR. |
| Principle / Technology | An engineered reverse transcriptase with improved template affinity, processivity and thermal stability synthesizes cDNA at elevated temperatures to reduce RNA secondary-structure interference. |
| Detection Method | Used upstream of endpoint PCR or qPCR analysis. |
| Sample Type | Animal, plant and viral RNA, including RNA containing polysaccharides, polyphenols or other common reverse-transcription inhibitors. |
| Performance Range / Specifications | 2,000 U or 10,000 U; reported half-life exceeds 240 minutes at 50°C and reaches approximately 60 minutes at 55°C; supports cDNA synthesis up to 20 kb. |
| Sensitivity / LOD | Demonstrated reverse transcription across RNA inputs from 1 µg to 1 pg in the reported evaluation. |
| Specificity | High-temperature reverse transcription improves access to structured RNA targets and supports specific downstream amplification. |
| Reaction Conditions / Protocol | Combine the enzyme with a compatible reverse-transcription buffer, dNTPs, RNase inhibitor and application-appropriate oligo(dT), random or gene-specific primers. Use elevated temperatures for structured RNA when required. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 2,000 U; 10,000 U. |
| Product Form | Thermostable reverse transcriptase enzyme solution. |
| Quality Control | Thermal stability, inhibitor tolerance, low-input sensitivity and long-cDNA synthesis are evaluated. |
| Key Features | High thermal stability; cDNA synthesis up to 20 kb; strong inhibitor tolerance; high sensitivity; suitable for structured RNA templates. |
| Compatibility | Compatible with first-strand cDNA synthesis, RNA-virus detection and two-step RT-qPCR workflows. |
| Application Notes / Precautions | Select primers according to RNA type. Random primers support broad RNA coverage, while oligo(dT) or gene-specific primers are preferred for long full-length cDNA targets. |
For research use only, not for clinical use.
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