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| Product Name | Taq DNA Polymerase |
| Catalog No. | IVDRM-0247 |
| Description | Taq DNA Polymerase is a thermostable recombinant DNA polymerase expressed from the thermophilic bacterium Thermus aquaticus, with a molecular weight of 94 kDa. The enzyme exhibits 5'→3' polymerase activity and 5'→3' exonuclease activity, but lacks 3'→5' exonuclease (proofreading) activity. Amplification products carry a 3'-dA overhang and can be used directly for TA cloning. |
| Intended Use | Raw material enzyme for routine PCR applications, including genotyping and colony PCR. |
| Principle / Technology | Thermostable DNA polymerase derived from Thermus aquaticus. The enzyme catalyzes template-directed DNA synthesis (5'→3' polymerase activity) and possesses 5'→3' exonuclease activity; the absence of 3'→5' proofreading activity results in non-templated addition of a single adenosine (3'-dA overhang) at the ends of amplification products. |
| Performance Range / Specifications | - Suitable for routine PCR such as genotyping and colony PCR. - PCR products carry 3'-dA overhangs and are directly compatible with TA cloning. |
| Reaction Conditions / Protocol | Unit activity is defined at 74 °C. Suitable for standard PCR thermal cycling protocols. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 1,000 U 10,000 U |
| Activity / Unit Definition | One unit (1 U) is defined as the amount of enzyme that incorporates 10 nmol of total nucleotides into acid-insoluble material within 30 min at 74 °C, using activated salmon sperm DNA as template/primer. |
| Molecular Weight | 94 kDa |
| Source / Origin | Recombinant expression of the thermostable DNA polymerase gene from the thermophilic bacterium Thermus aquaticus. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Compatibility | Amplification products carry 3'-dA overhangs and are directly compatible with TA cloning vectors. |
| Application Notes / Precautions | - Recommended for routine PCR applications such as genotyping and colony PCR. - The enzyme has no 3'→5' exonuclease (proofreading) activity; for applications requiring high fidelity, use a proofreading polymerase instead. - PCR products contain 3'-dA overhangs and can be cloned directly into T vectors for TA cloning. |
For research use only, not for clinical use.
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