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| Product Name | T4 UvsY Protein (2 μg/μL) |
| Catalog No. | IVDRM-0256 |
| Description | T4 UvsY Protein is a recombinant regulatory protein derived from bacteriophage T4 that promotes the activity of T4 UvsX Recombinase during homologous recombination. When T4 UvsX Recombinase searches for homologous sequences, it must compete with single-stranded DNA-binding proteins pre-bound to the ssDNA; T4 UvsY Protein facilitates this competition and assists UvsX binding to single-stranded DNA. T4 UvsY Protein has a molecular weight of ~16 kDa. It enhances the DNA-dependent ATPase activity of T4 UvsX Recombinase and lowers the minimum recombinase concentration required for activity, thereby promoting strand displacement. It promotes invasion of the ssDNA–SSB complex by T4 UvsX Recombinase, leading to release of the single-stranded DNA-binding protein and facilitating UvsX loading onto ssDNA. The protein has no nuclease activity. |
| Intended Use | Recombinase loading/accessory factor for isothermal amplification using recombinase polymerase amplification (RPA) technology. |
| Principle / Technology | T4 UvsY acts as the recombinase loading factor (mediator) in RPA: it promotes T4 UvsX Recombinase invasion of ssDNA–single-stranded-binding-protein complexes, displacing SSB and facilitating UvsX nucleoprotein filament formation on ssDNA primers. It also stimulates the DNA-dependent ATPase activity of UvsX and reduces the minimum UvsX concentration required, thereby promoting homology search and strand displacement. |
| Detection Method | Isothermal amplification (RPA) |
| Performance Range / Specifications | Mainly used in isothermal recombinase polymerase amplification (RPA) technology. Storage buffer composition: 50 mM Tris-HCl, 150 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25 °C. |
| Components / Formulation | 50 mM Tris-HCl, 150 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, pH 7.5 @ 25 °C |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years when stored at -25 °C to -15 °C. |
| Package Specifications | 100 μg; 500 μg; 2 mg; 10 mg |
| Product Form | Liquid protein solution in 50% glycerol storage buffer |
| Quality Control | No nuclease activity. |
| Concentration | 2 μg/μL |
| Molecular Weight | ~16 kDa |
| Source / Origin | Bacteriophage T4-derived recombinant regulatory protein. |
| pH Range / Optimal pH | Supplied in buffer at pH 7.5 @ 25 °C |
| Expiration Date / Stability | 2-year shelf life at -25 °C to -15 °C. |
| Compatibility | Used together with T4 UvsX Recombinase and single-stranded DNA-binding protein in RPA reaction systems. |
| Application Notes / Precautions | Supplied in 50% glycerol storage buffer; avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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