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| Product Name | T4 UvsX Recombinase (2 μg/μL) |
| Catalog No. | IVDRM-0255 |
| Description | T4 UvsX Recombinase is derived from bacteriophage T4 and is a homolog of the RecA/Rad51 family, playing an important role in the repair of double-strand DNA breaks and the restart of replication forks. Together with other DNA-binding proteins or accessory factors, T4 UvsX Recombinase forms nucleoprotein complexes with single-stranded DNA; these complexes search for complementary regions in the target DNA and hybridize to complete the strand displacement reaction. The enzyme has no nuclease activity. |
| Intended Use | Core recombinase raw material for isothermal amplification using recombinase polymerase amplification (RPA) technology. |
| Principle / Technology | In RPA, T4 UvsX Recombinase (the RecA/Rad51 family recombinase) binds single-stranded DNA primers in the presence of accessory proteins/loading factors to form a nucleoprotein filament. The filament scans double-stranded target DNA for homologous sequences, invades the duplex and initiates strand exchange, enabling strand displacement and primer extension at constant temperature. |
| Detection Method | Isothermal amplification (RPA) |
| Performance Range / Specifications | Mainly used in isothermal recombinase polymerase amplification (RPA) technology. Storage buffer composition: 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% glycerol, pH 7.5 @ 25 °C. The storage buffer contains 20% glycerol; aliquoting is recommended to avoid repeated freeze-thaw cycles. |
| Components / Formulation | 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% glycerol, pH 7.5 @ 25 °C |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years when stored at -25 °C to -15 °C. |
| Package Specifications | 100 μg; 1 mg; 5 mg; 20 mg |
| Product Form | Liquid protein solution in 20% glycerol storage buffer |
| Quality Control | No nuclease activity. |
| Concentration | 2 μg/μL |
| Source / Origin | Bacteriophage T4-derived recombinase (RecA/Rad51 family homolog), recombinantly expressed. |
| pH Range / Optimal pH | Supplied in buffer at pH 7.5 @ 25 °C |
| Expiration Date / Stability | 2-year shelf life at -25 °C to -15 °C. |
| Compatibility | Works together with single-stranded DNA-binding proteins and loading factors (e.g., T4 UvsY Protein) in RPA reaction systems. |
| Application Notes / Precautions | The storage buffer contains 20% glycerol; aliquot upon receipt and store separately to avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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