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| Product Name | T4 Gene 32 Protein (gp32, 10 μg/μL) |
| Catalog No. | IVDRM-0272 |
| Description | T4 Gene 32 Protein (gp32, 10 μg/μL) is a single-stranded DNA (ssDNA) binding protein encoded by gene 32 of bacteriophage T4 and is essential for T4 phage DNA replication and repair. It binds cooperatively to and stabilizes transiently formed ssDNA regions, playing an important structural role during T4 phage DNA replication. The protein is also widely used to stabilize and label ssDNA regions for electron microscopy studies of intracellular DNA structure. In recombinase polymerase amplification (RPA), gp32 binds the displaced strand generated during the reaction and prevents its further displacement, thereby supporting efficient amplification. |
| Intended Use | Core single-enzyme raw material for recombinase polymerase amplification (RPA); also suitable for stabilizing and labeling ssDNA regions for structural DNA studies. |
| Principle / Technology | In RPA, recombinase-primer filaments invade double-stranded DNA and displace one strand; gp32 is a cooperative ssDNA binding protein that binds and stabilizes the displaced single strand, preventing it from being further displaced or re-annealing, and thereby enabling the strand-displacing polymerase to extend the primers and drive exponential isothermal amplification. |
| Detection Method | Recombinase polymerase amplification (RPA) |
| Sample Type | DNA |
| Performance Range / Specifications | Concentration: 10 μg/μL Increases the yield and efficiency of reverse transcriptase in RT-PCR Promotes restriction endonuclease digestion Enhances T4 DNA polymerase activity Increases PCR yield Stabilizes and labels single-stranded DNA regions for intracellular DNA structure analysis |
| Components / Formulation | Supplied as a concentrated protein solution; formulation details available on request. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years |
| Package Specifications | 250 μg; 5 mg |
| Product Form | Liquid |
| Concentration | 10 μg/μL |
| Source / Origin | Recombinantly expressed protein encoded by gene 32 of bacteriophage T4. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15°C. |
| Regulatory / Compliance | For research use and IVD raw material manufacturing. |
| Compatibility | Compatible with RPA reaction systems; functional in RT-PCR, PCR, restriction digestion, and T4 DNA polymerase reactions. |
For research use only, not for clinical use.
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