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| Product Name | T4 DNA Ligase |
| Catalog No. | IEC-HMM-0083 |
| Description | A highly purified T4 DNA ligase preparation supplied with a 10× reaction buffer for efficient joining of compatible nucleic acid termini. |
| Intended Use | Restriction-fragment ligation, TA cloning, sticky- and blunt-end DNA ligation, RNA 3′-end labeling, RNA or DNA oligonucleotide circularization and long-cDNA cloning. |
| Principle / Technology | Catalyzes phosphodiester-bond formation between adjacent 5′-phosphate and 3′-hydroxyl termini in double-stranded DNA and in selected DNA or RNA duplex structures. |
| Sample Type | Double-stranded DNA with sticky or blunt ends and selected RNA or DNA/RNA duplex substrates. |
| Specificity | Does not ligate fully single-stranded nucleic acids. |
| Components / Formulation | 10× ligase buffer, 1 mL; T4 DNA ligase at 400 U/μL, 100 μL. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 40,000 U. |
| Product Form | DNA ligase enzyme solution with reaction buffer. |
| Quality Control | Reported purity exceeds 99%, with no detectable residual exogenous nuclease activity. |
| Key Features | High purity; broad cohesive- and blunt-end compatibility; classical optimized buffer; suitable for DNA and selected RNA ligation applications. |
| Purity | >99%. |
| Concentration | 400 U/μL. |
| Activity / Unit Definition | 40,000 U total activity per package. |
| Application Notes / Precautions | A small amount of precipitate may appear when the buffer is thawed; invert gently to mix before use. |
For research use only, not for clinical use.
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