- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Super Reverse Transcriptase (200 U/μL) |
| Catalog No. | IVDRM-0243 |
| Description | This product is a new-generation reverse transcriptase obtained by directed evolution of M-MLV reverse transcriptase using a proprietary enzyme engineering platform. The enzyme lacks RNase H activity, and its thermostability is greatly improved, tolerating reaction temperatures of up to 70 °C. It is highly suitable for efficient reverse transcription of RNA templates with complex secondary structures. |
| Intended Use | Raw material enzyme for in vitro diagnostic reagent development, particularly high-temperature reverse transcription and RT-LAMP (isothermal amplification) applications. |
| Principle / Technology | Directed evolution of M-MLV reverse transcriptase yields an RNase H-deficient enzyme with greatly enhanced thermostability, enabling reverse transcription at temperatures up to 70 °C. |
| Detection Method | Reverse transcription coupled with PCR or isothermal amplification (RT-LAMP) |
| Sample Type | RNA templates, including RNA with complex secondary structures |
| Performance Range / Specifications | Supports reverse transcription at up to 70 °C. Suitable for high-temperature RT-LAMP reactions. |
| Reaction Conditions / Protocol | Reverse transcription can be performed at elevated temperatures up to 70 °C, helping to resolve RNA templates with complex secondary structures. |
| Components / Formulation | RNase H-deficient thermostable reverse transcriptase supplied at 200 U/uL. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 18 months |
| Package Specifications | 10000 U; 50000 U |
| Product Form | Liquid enzyme solution, 200 U/uL |
| Concentration | 200 U/uL |
| Source / Origin | Recombinant enzyme obtained by directed evolution of M-MLV (Moloney murine leukemia virus) reverse transcriptase |
| Expiration Date / Stability | Stable for 18 months at -25 to -15 °C. |
| Compatibility | Compatible with high-temperature RT-LAMP and other isothermal amplification workflows. |
| Application Notes / Precautions | Lacks RNase H activity, preserving RNA templates during cDNA synthesis; high reaction temperatures (up to 70 °C) are recommended for RNA templates with stable secondary structures. |
| Heat Inactivation | Highly thermostable enzyme; supports reverse transcription at reaction temperatures up to 70 °C. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
