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| Product Name | Super Bst DNA Polymerase (2,000 U/μL) |
| Catalog No. | IVDRM-0234 |
| Description | Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp. DNA Polymerase I and genetically engineered to remove its 5'→3' exonuclease activity. The enzyme exhibits strong 5'→3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it well suited for contamination-controlled isothermal amplification reactions such as LAMP and CPA. Compared with standard Bst Plus polymerases, this enzyme offers enhanced thermostability, which improves amplification performance. It is suitable for low-template and high-temperature LAMP reaction systems, effectively increasing detection specificity and shortening time-to-result. Its heat-resistant property also supports the development of hot-start Bst formulations for further improvement of assay specificity. |
| Intended Use | Raw material enzyme for isothermal amplification-based molecular diagnostics, including LAMP (loop-mediated isothermal amplification) and CPA (crossing priming amplification). Particularly suited for low-template reactions, high-temperature LAMP systems, and the development of hot-start Bst formulations. |
| Principle / Technology | Engineered DNA Polymerase I from Thermophilic Geobacillus sp. with the 5'→3' exonuclease domain removed. The enzyme polymerizes DNA with strong 5'→3' polymerase and strand displacement activities, enabling amplification at a constant temperature without thermal cycling. |
| Performance Range / Specifications | Enhanced thermostability supports amplification of complex templates and increases reaction specificity at elevated temperatures (up to 70 °C). |
| Reaction Conditions / Protocol | Suitable for high-temperature isothermal amplification (e.g., LAMP at up to 70 °C). Compatible with low-template reaction systems. |
| Storage Conditions | Store at -25 °C to -15 °C. |
| Shelf Life | 2 years |
| Package Specifications | 20,000 U 100,000 U 200,000 U |
| Concentration | 2,000 U/uL |
| Source / Origin | Recombinant, genetically engineered Thermophilic Geobacillus sp. DNA Polymerase I with the 5'→3' exonuclease activity removed. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 °C to -15 °C. |
| Compatibility | Tolerates dUTP, making it compatible with dUTP/UDG carryover contamination control systems for isothermal amplification. |
| Application Notes / Precautions | - Recommended for LAMP, CPA, and other isothermal amplification reactions. - The enhanced thermostability allows higher reaction temperatures, which improves specificity and facilitates amplification of complex templates. - Suitable for low-template systems; helps shorten detection time. - Can be used to develop hot-start Bst formulations to further improve detection specificity. |
For research use only, not for clinical use.
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