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| Product Name | Super Bst DNA Polymerase (120 U/μL) |
| Catalog No. | IVDRM-0270 |
| Description | Super Bst DNA Polymerase (120 U/μL) is an engineered derivative of Thermophilic Geobacillus sp. DNA Polymerase I in which the 5'→3' exonuclease activity has been removed. The enzyme exhibits strong 5'→3' DNA polymerase activity, robust strand-displacement activity, and tolerance to dUTP, making it well suited for contamination-controlled isothermal amplification reactions such as LAMP and CPA. Its enhanced thermostability supports amplification at up to 70°C, improves performance with low-concentration templates, shortens time-to-result, and provides a foundation for developing hot-start Bst formulations that further increase detection specificity. |
| Intended Use | Suitable for a variety of isothermal amplification reactions, including LAMP, CPA, and RCA; supports 70°C LAMP and, in combination with a thermostable MMLV reverse transcriptase, 70°C RT-LAMP. |
| Principle / Technology | Engineered Bst-family DNA polymerase with strong 5'→3' polymerase and strand-displacement activity (5'→3' exonuclease deficient), enabling rapid isothermal amplification of DNA targets at a constant temperature, including elevated-temperature (70°C) LAMP formats. dUTP tolerance enables UDG-mediated carryover contamination control. |
| Detection Method | Isothermal amplification (LAMP, CPA, RCA, RT-LAMP) |
| Sample Type | DNA templates, including low-concentration samples |
| Performance Range / Specifications | Concentration: 120 U/μL Compatible with LAMP, CPA, RCA and other isothermal amplification reactions High thermostability: enables LAMP at 70°C, or 70°C RT-LAMP when paired with a thermostable MMLV reverse transcriptase |
| Sensitivity / LOD | Supports amplification of low-concentration templates; increasing reaction temperature to 70°C significantly shortens Ct values and further improves assay sensitivity. |
| Specificity | Elevated-temperature amplification (70°C) increases detection specificity; dUTP tolerance allows use in UDG-based anti-contamination systems. |
| Reaction Conditions / Protocol | Typical LAMP reaction temperature: 65–70°C. For RT-LAMP at 70°C, combine with a thermostable MMLV reverse transcriptase. |
| Components / Formulation | Supplied as a concentrated enzyme solution; formulation details available on request. |
| Storage Conditions | Store at -25 to -15°C. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years |
| Package Specifications | 6,000 U; 24,000 U; 120,000 U |
| Product Form | Liquid |
| Quality Control | Amplification performance verified by functional isothermal amplification testing. |
| Concentration | 120 U/μL |
| Source / Origin | Recombinantly expressed enzyme engineered from Thermophilic Geobacillus sp. DNA Polymerase I, with the 5'→3' exonuclease activity removed. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15°C; avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use and IVD raw material manufacturing. |
| Compatibility | Compatible with LAMP, CPA, and RCA buffer systems; dUTP-tolerant for use with UDG anti-contamination workflows; pairs with thermostable MMLV reverse transcriptase for RT-LAMP. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles. Suitable for developing hot-start Bst formulations to further enhance specificity. |
For research use only, not for clinical use.
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