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| Product Name | Sarcosine Oxidase (SOX) |
| Catalog No. | IEC-HMM-0130 |
| Description | Alta DiagnoTech Sarcosine Oxidase (SOX) is a recombinant enzyme supplied as white lyophilized powder. The enzyme has a molecular weight of 44 kDa (SDS-PAGE) by SDS-PAGE. |
| Intended Use | For enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase. |
| Principle / Technology | One unit deaminates 1 µmol of sarcosine per minute at pH 8.0 and 37°C. |
| Performance Range / Specifications | EC 1.5.3.1; CAS 9029-22-5; Specific activity: ≥25 U/mg protein |
| Reaction Conditions / Protocol | Optimum pH: 7.0-8.5; Optimum temperature: 50°C; pH stability: pH 7.0-10.5 at 25°C for 24 hours; Thermal stability: Below 50°C at pH 8.0 for 30 minutes; Inhibitors: Ag+, Hg2+, and Cu2+ |
| Components / Formulation | White lyophilized powder |
| Storage Conditions | Store at or below -20°C. |
| Shelf Life | 18 months at -20°C. |
| Product Form | White lyophilized powder |
| Key Features | White lyophilized powder; Molecular weight: 44 kDa (SDS-PAGE); EC 1.5.3.1; Specific activity: ≥25 U/mg protein; Optimum pH: 7.0-8.5 |
| Activity / Unit Definition | Specific activity: ≥25 U/mg protein; Unit definition: One unit deaminates 1 µmol of sarcosine per minute at pH 8.0 and 37°C. |
| Molecular Weight | 44 kDa (SDS-PAGE) |
| Source / Origin | Recombinant |
| pH Range / Optimal pH | 7.0-8.5 |
| Expiration Date / Stability | pH 7.0-10.5 at 25°C for 24 hours; Below 50°C at pH 8.0 for 30 minutes |
| Compatibility | Creatinine amidohydrolase and creatine amidinohydrolase |
| Alternative Names / Synonyms | Sarcosine Oxidase (SOX); SOX |
For research use only, not for clinical use.
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